网状壳聚糖固定化黄鱼中甲醛残留的条件优化

Immobilization of Formaldehyde Residue in Yellow Croaker on Reticular Chitosan

摘要:

以壳聚糖为载体，甲醛浸泡过的黄鱼、人血清白蛋白为材料，研究一种壳聚糖法固定黄鱼中甲醛的方法。本文对影响固定化的关键因素：戊二醛浓度、甲醛-人血清白蛋白（FA-HSA）用量、吸附温度等展开研究，以FA-HSA吸附率为指标，分别进行单因素实验和正交实验，探索其固定化最佳条件。实验结果表明，当戊二醛浓度为5%，FA-HSA用量为14 mL，吸附温度为42 ℃，吸附时间为4 h时，FA-HSA的吸附量为最大值，且达到59.2%。

Abstract:

With Chitosan as a carrier, a method for immobilizing formaldehyde residue in yellow croaker was developed. Effects of several key factors, including glutaraldehyde concentration, the amount of FA-HSA, adsorption temperature and so on, on immobilization were investigated taking the adsorption rate of FA-HSA as the indicators. Through single factor experiments and orthogonal test, the best condition of formaldehyde immobilization on chitosan were determined as: glutaraldehyde concentration 5%, the amount of FA-HSA 14mL, adsorption temperature 42 ℃ and the adsorption time 4 h. Under the optimum immobilization conditions, the adsorption rate of FA-HSA reached the highest rate of 59.2%.