高产淀粉酶和蛋白酶的黑曲霉菌株的发酵条件优化

Optimization of Fermentation Conditions for the Produciton of Amylase and Protease by a Strain of Aspergillus niger

摘要:

本文分离筛选到一株高产蛋白酶和淀粉酶黑曲霉菌株A020，确定该菌的最佳产酶培养基为：香蕉皮基础培养基中添加1%蛋白胨、1%麦芽糖和3×10-3 mol/L NaCl；最佳发酵条件为：30 ℃、pH 6.5、装液量150 mL/500 mL。在此条件下，150 r/min摇瓶培养4 d，测得淀粉酶、蛋白酶酶活分别为1278 U/mL和918 U/mL，淀粉酶和蛋白酶的酶活分别比优化前提高了68.2%、41.2%。

Abstract:

A strain A020 of Aspergillus niger with high productivity of amylase and protease was isolated. The optimum compositions of fermentation medium were as followed: 1% peptone, 1% maltose and 3×10-3 mol/L NaCl in banana peel basic medium. And the optimum fermentation conditions were achieved as follows: temperature 30 ℃, initial pH 6.5 and medium volume 150 mL in 500 mL flask. Under these conditions, the activities of amylase and protease and cultured for 4 d at 150 r/min were 1278 U/mL and 918 U/mL, respectively. The amylase and protease activities increased by 68.2% and 41.2%, respectively.