阴离子交换层析法分离纯化花生主要过敏原Ara h1的研究

Purification of Peanut Allergen Ara h1 with Anion-exchange Chromatography

摘要:

Ara h1蛋白是花生的主要过敏原蛋白。本研究从天然的花生提取花生蛋白质，通过硫酸铵分级沉淀及阴离子交换层析法纯化花生主要过敏原Ara h1。通过聚丙烯酰胺凝胶电泳分析蛋白纯度，结合免疫印迹实验对Ara h1免疫活性进行鉴定。结果表明：采用阴离子交换层析法纯化出的Ara h1纯度达到90%以上。得率为23.1%。免疫印迹实验表明，此方法纯化出来的Ara h1仍具有免疫原性，能跟花生过敏病人血清特异结合。

Abstract:

Protien Ara h1 is a major allergen in peanut. In order to obtain the natural peanut allergen Ara h1, ammonium sulfate precipitation and anion-exchange chromatography were used for purification. The analysis and identification of peanut allergens was carried out using SDS-PAGE and Western blot. The results showed that the purity of Ara h1 was more than 90 percent and the recovery accounted 23.1% of total Ara h1 with the anion-exchange chromatography. The Ara h1 purfied by anion-exchange chromatography had immunocompetence. It can combine to peanut allergy patients serum with high specificity.