拟黑多刺蚁乙醇浸提液的理化性质及抗氧化活性研究

Physical-chemical and Antioxidant Properties of the Alcohol Extracts of Polyrhachis vicina Roger

摘要:

采用Alcalase 2.4 L水解拟黑多刺蚁，以抗氧化活性为指标，并结合蛋白质利用率、多肽含量等指标比较拟黑多刺蚁及其酶解物的醇提物的理化性质及抗氧化性的区别。结果表明，在乙醇浓度介于35~75%，酶解拟黑多刺蚁醇提物的氨基酸和多肽含量分别介于4.20~3.19 mg/g和5.19~4.07 mg/mL，是没有经酶解的拟黑多刺蚁醇提物的2倍左右。酶解提高了拟黑多刺蚁醇提物的抗氧化性，在乙醇浓度为55%时，其超氧自由基、DPPH自由基、羟基自由基清除能力和还原力的IC50分别为0.276、0.106、0.279和0.227 mg/mL。同时，酶解拟黑多刺蚁醇提物的重要微量元素Zn、Mn和Ca也有显著提高。

Abstract:

Polyrhachis vicina Roger was hydrolyzed by alcalase 2.4L. The differences in physical-chemical and antioxidant activity of the extracts and hydrolysatge of Polyrhachis vicina Roger were evaluated. The results showed that, when alcohol concentration was between 35~75%, the amino acid and peptide contents of the extracts of hydrolyzed Polyrhachis vicina Roger were 4.20~3.19 mg/g and 5.19~4.07 mg/mL respectively, almost 2 times higher than that of the unhydrolyzed. The enzymatic hydrolysis could improve the antioxidant activity of the extracts. When alcohol concentration was of 55%, the IC50 of superoxide radical, DPPH? and hydroxyl radical and reducing power were 0.276, 0.106, 0.279 and 0.227 mg/mL, respectively. Furthermore, the important trace minerals such as Zn, Mn and Ca of the alcohol extract of enzymatic hydrolysates were increased significantly.