全缘马尾藻褐藻多酚的抗氧化和抗肿瘤细胞增殖作用研究

Antioxidant Activity and Antiproliferation Effect on Tumor Cells of Phlorotannins from Sargassum integerrimum

摘要:

检测全缘马尾藻褐藻多酚提取物(IPTs)的抗氧化和抗肿瘤细胞增殖作用。通过萃取和超滤获得4种IPTs提取物-IPTa1、IPTa2、IPTe1和IPTe2，总酚含量分别为1.16、0.6、0.25和0.34 mg没食子酸（GA）/g海藻干基，1.5 mg/L时的总抗氧化能力（TCA）分别相当于1.724、1.379、1.833、0.695 mg/L的Fe2+还原能力；MTT结果显示，4.4 μg/mL时，4种IPTs提取物作用sw579细胞24 h、48 h、72 h后，除了IPTa2外，IPTa1、IPTe1和IPTe2均以时间依赖方式显著抑制肿瘤细胞的生长；0.3125~5 μg/mL浓度范围内，IPTa1和IPTe1的抑制率与作用时间和浓度呈正相关，最高抑制率达90%以上。

Abstract:

To investigate antioxidant activity and antiproliferation effect of phlorotannins extraction from Sargassum integerrimum (IPTs) IPTs was prepared by solvent extraction and ultrafiltration. Four extracts named IPTa1, IPTa2, IPTe1 and IPTe2 were attained. Total phenolic contents of the four IPTs were 1.16, 0.6, 0.25 and 0.34 mg GA/g algae dry base. Total antioxidant capacities of the four extracts at 1.5 mg/L were equal to reduction capacities of Fe2+at 1.724, 1.379, 1.833 and 0.695 mg/L, respectively. MTT results showed that the four extracts except IPTa2 inhibited significantly proliferation of thyroid squamous cell carcinoma cells (sw579) at the concentration of 4.4 μg/mL and their inhibition rates increased in a time-dependant manner within 72 hours. Inhibition rates of IPTa1 and IPTe1 had a positive correlation with time and concentration at range of 0.3125~5 μg/mL and the highest inhibition rate was up to 90%.