本试验探索了蝇蛆壳制备壳聚糖的最佳工艺条件。以蝇蛆壳中甲壳素为原料，采用碱液法制备壳聚糖。通过单因素试验考察了碱液浓度、液料比、反应时间对壳聚糖脱乙酰度的影响；根据Box-Benhnken中心组合试验设计原理，采用三因素三水平响应面分析法优化脱乙酰基反应条件，依据回归分析确定工艺条件的主要影响因素，以壳聚糖脱乙酰度为响应值作响应面和等值线图。通过分析各个因素的显著性和交互作用，得出脱乙酰基反应的最佳工艺条件为：液料比57.49:1 (V/m)，碱液浓度50%，反应时间7.3 h。在此条件下所得壳聚糖的脱乙酰度理论值为83.18%，实测值为79.45%，并得到白色粉末状壳聚糖。

To optimize the preparation condition for the chitosan from Flies maggots, chitin from Flies maggots shell was used as raw material, and alkali liquor method was used to prepare chitosan. Effects of sodium hydroxide concentration, ratio of liquid to material and reaction time on the deacelation degree of chitosan were investigated respectively by single-factor tests, and then the process conditions of the deacelation was optimized through response surface methodology with three factors and three levels based on the principle of Box-Benhnken design. The results showed that the optimum conditions of the deacelation reaction were as follows: ratio of liquid to material 57.49:1, sodium hydroxide concentration 50%, and reaction time 7.3 h. Under these conditions, the theoretical value of the chitosan deacelation degree was 83.18%, and 79.45%, respectively. The chitosan product was a white powder.

黑龙江省研究生创新项目（YJSCX2012-388HLJ）