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[摘要]
研究了富硒大豆低聚肽在抗氧化方面的作用。将3周龄SD雄性大鼠90只,随机分为6组,即Ⅰ(对照组)、Ⅱ(大豆蛋白组)、Ⅲ(大豆多肽组)、Ⅳ(亚硒酸钠组)、Ⅴ(大豆低聚肽组)、Ⅵ(富硒大豆低聚肽组),在实验环境下,对各组饲喂低硒基础饲料的同时,对第Ⅱ组口腔灌喂大豆蛋白,对第Ⅲ组口腔灌喂大豆多肽,对第Ⅳ组口腔灌喂亚硒酸钠,对第Ⅴ组口腔灌喂大豆低聚肽,对第Ⅵ组口腔灌喂富硒大豆低聚肽,第Ⅰ组口腔灌喂同体积的饮用水。饲喂4周,处死后分别测定各实验大鼠血清和肝脏中的MDA(丙二醛)含量、GSH-Px和SOD活性。结果显示:第Ⅱ、Ⅲ、Ⅴ、Ⅳ、Ⅵ组均能降低血清和肝脏中MDA的含量,提高GSH-Px和SOD活性,但第Ⅱ、Ⅲ组作用不显著,第Ⅴ组作用显著,第Ⅳ、Ⅵ组作用极显著。各组样品对血清和肝脏中MDA含量和GSH-Px活性的影响效果趋于一致,但对血清SOD活性的影响明显强于对肝脏SOD活性的影响,实验结果说明富硒大豆低聚肽具有抗氧化功能,这种功能主要通过降低大鼠血清和肝脏中MDA的含量,提高GSH-Px和SOD活性来实现,富硒大豆低聚肽的抗氧化功能要强于无机硒,明显强于不含硒的大豆低聚肽,推测富硒大豆低聚肽中发挥抗氧化功能的主要是微量元素硒。
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[Abstract]
The antioxidation effect of Se-riched soybean oligopeptides were researched. 3 weeks of age 90 male SD rats were randomly divided into 6 groups, namely Ⅰ(control group ), II ( soy protein ), III (soy polypeptides ), IV (sodium selenite), V ( soy oligopeptides) and VI (Se-riched soy oligopeptides), in the experimental environment, for each of the groups fed the low selenium based feed at the same time, the second group of oral feeding of soybean protein, group Ⅲoral feeding of soybean polypeptide, the IV group of oral feeding of sodium selenite, the Article V group oral feeding soybean oligopeptides, on the sixth group oral feeding soybean oligopeptides, group 1oral feeding with the same volume of drinking water. Feeding for 4 weeks, serum and liver MDA content, GSH-Px and SOD activity of all experimental rats were measured. Results showed that the II, III, IV, V and VI groups were decreased in serum and liver MDA content, GSH-Px and SOD activity increased, but the II and Ⅲ group had no significant effect and the V group had notable effect. The IV, VI group had extremely significant effect. Each sample on blood serum and liver MDA content and GSH-Px activity effect tends to be consistent, but the serum SOD activity was stronger than hepatic SOD activity. Those meant that the Se-riched soybean oligopeptides had antioxidant functions, and the function was stronger than inorganic selenium, and obviously stronger than soybean oligopeptides which had not selenium. The Se-riched soybean oligopeptides played the main antioxidant function and was the trace element selenium.
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