油菜花粉酶解肽对60Coγ辐射损伤小鼠造血功能的影响

Effects of Peptide from Rape Pollen Glutelin on Hemopoietic Function Promotion of Mice Irradiated by 60Coγ

摘要:

探讨油菜花粉酶解肽对8 Gy的60Coγ辐射损伤小鼠造血功能的影响，为开发安全无毒有效的抗辐射药物和提高花粉附加值提供依据。小鼠随机分为空白组、照射对照组、油菜花粉酶解肽低、中、高剂量组，样品采用灌胃方式给予实验小鼠，连续灌胃30 d后，接受8 Gy 60Coγ射线一次性辐射，照射后定期对小鼠尾静脉取血并做血象分析；辐射后20 d，测定骨髓细胞微核率及DNA含量、肝脏SOD活性、血清MDA值。与对照组比较，油菜花粉酶解肽能一定程度上改善外周血象指标（白细胞、红细胞、血小板数、血红蛋白），降低骨髓细胞微核率，提高小鼠骨髓细胞DNA含量、提高SOD的酶活和降低血清MDA含量，且在各剂量组中高剂量组效果教好。油菜花粉酶解肽对辐射损伤小鼠的造血功能具有一定的防护作用。

Abstract:

To evaluate the protective effects of peptide from rape pollen glutelin on hemopoietic function promotion of irradiated mice in 60Coγ, and to provide theoretical evidence for developing the safe and harmless antiradiation medicine and improving added value of pollen. The mices were divid The protective effects of peptide from rape pollen glutelin (PRPG ) on hemopoietic function promotion of irradiated mice in 60Coγ were evaluated in this research to provide theoretical evidence for developing the safe and harmless antiradiation medicine and improving added value of pollen. The mice were divided into normal control group, irradiating control group, low, medium, and high dose group. For 30 days of being intragastricaly administrated, all mice were irradiated by 8 Gy 60Co-γ-ray except normal control group. Then the peripheral blood WBC, RBC, PLT and HGB of mices were analysis on fixed days. 20 days of radiation later，the activity of SOD in hepatic tissue and MDA content in serum were studied, and the DNA content of bone narrow , the rate of nucleated bone narrow cell were detected. Comparing with the model group, the mice of PRPG peripheral blood WBC, RBC, PLT and HGB were higher. The rate of nucleated bone narrow cell was decreased,. The DNA content of bone narrow and the activities of SOD in liver were increased, and MDA in serum were decreased. And the 100 mg / kg.bw dose group was found to be the best. PRPG had protective effects on hemopoietic function promotion of irradiated mice in 60Coγ.