双水相-超声波提取榴莲果皮总黄酮的工艺研究

Study on Extraction Technology of the Flavonoids Ultrasonic by Wave and Aqueous Two-phase System in the Durio Zibethinus Peel

摘要:

在乙醇-硫酸铵双水相中，采用超声波法提取了榴莲皮中的总黄酮。考查了乙醇浓度、硫酸铵用量、料液比、超声功率、超声时间、提取温度对总黄酮提取率的影响。通过L9 (34)正交试验设计，确立了榴莲果皮中总黄酮提取最佳工艺条件为：60%乙醇浓度，料液比为1:25，超声波功率280 W，超声时间50 min，提取温度70 ℃。按照最优条件提取，总黄酮得率达3.26%。

Abstract:

The flavonoids were extracted in the ethanol-(NH4)2SO4 aqueous two-phase system by ultrasonic cooperation. The ethanol concentration, the amount of the (NH4)2SO4, the solid-liquid ratio, the ultrasonic power, the ultrasonic time and the extraction temperature were discussed by the single factor experiment. The optimum extraction conditions were optimized by the L9 (34) orthogonal test. The optimum extraction conditions of the flavonoids were as follows: the ethanol concentration 60%, the solid-liquid ratio 1:25, the ultrasonic power 280 W, the ultrasonic time 50, and the extraction temperature 70 ℃.The yield of the flavonoid was reached 3.26% at the optimum conditions.