早花及晚花灰毡毛忍冬中绿原酸含量分析

Analysis on the Content of Chlorogenic Acid from Early-flowering and Later-flowering Lonicera Macranthoides

摘要:

研究早花和晚花灰毡毛忍冬花蕾及叶中绿原酸含量，筛选适宜贵州北部推广种植优良品种，采用超声波辅助提取灰毡毛忍冬中的绿原酸，高效液相色谱法测定其含量，色谱条件为：Diamonsil柱 (5 μm, C18(2), 250 mm× 4.6 mm)，甲醇/乙腈(5:11)-0.1%磷酸=20:80，流速1.0 mL/min，柱温40 ℃，检测波长325 nm。结果表明早花品种花蕾中绿原酸含量为2.36%~3.44%，叶中绿原酸含量为0.49%~1.11%；晚花花蕾中绿原酸含量为2.60%~4.07%，叶中绿原酸含量为1.92%~2.83%。因此晚花灰毡毛忍冬是一种富绿原酸育种系。

Abstract:

To explore suitable planting fine Lonicera macranthoides varieties in north Guizhou, the content of chlorogenic acid in flower buds and leaves of early-flowering and later-flowering varieties were researched. Chlorogenic acid were obtained with ultrasonnic wave-assisted extraction technique, and analyzed using HPLC methods with operating condition as follows: Diamonsil (5 μm, C18(2), 250 mm×4.6 mm),the volume flow 1.0mL/min，the column temperature 40℃ and the detection wavelength 325nm. The mobile phase methanol/acetonitrile (5:11)-0.1% phosphoric acid=20:80. The result showed that the content of chlorogenic acid ranged from 2.36% to 3.44% in flower buds and from 0.49% to 1.11% in leaves of early-flowering Lonicera macranthoides. In flower buds and leaves of later-flowering one, it as in the range of 2.60%~4.07% and 1.92%~2.83%, respectively. It was concluded that the later-flowering Lonicera macranthoide was a rich-chlorogenic acid breeding strain.