本文研究了盐酸-乙醇等电点法脱除油茶籽多糖中蛋白质的方法，此法在盐酸-等电点法的基础上，添加一定量的乙醇溶液，使之更有利于蛋白质的沉淀脱除。通过单因素试验及正交试验，确定蛋白质脱除的最佳条件为：pH 3.4，乙醇添加量为13%，静止时间为3 d，可以使蛋白质脱除率达到92.68%，多糖保留率为61.78%。与传统的三氯乙酸法和Sevage法进行比较，此法更有效且更安全。

This paper studied a new type of proteins removal method from camellia polysaccharides, hydrochloric acid-ethanol isoelectric point method.On the basis of hydrochloric acid isoelectric point method, the new method adding a certain amount of ethyl alcohol into suspension is more effective than the traditional methods to precipitate proteins. Through single factor experiments and orthogonal test, the optimum condition was found as follows: pH 3.4, ethyl alcohol adding 13% and standing time 3 days, under which protein removal rate and polysaccharides yield reached 92.68% and 61.78, respectively. Compared with the traditional method of trichloroacetic acid (TDA) and Sevage, it was more effective and more safe.

湖南省科技厅重大专项(2009FJ1006-3)