以低聚壳聚糖作为氨基供体分别与提供羰基的葡萄糖和麦芽糖进行美拉德反应（氨基与羰基的物质量比均为1:1），醇沉法提取低聚壳聚糖美拉德反应衍生物CG和CM。对两种衍生物进行红外表征和分子量测定，并研究其抗氧化性能和抑菌性能。结果显示：两种衍生物均保留着低聚壳聚糖的特征吸收峰；其对O2.-、.OH及DPPH的清除能力以及还原能力和对大肠杆菌、铜绿假单胞菌和副溶血性弧菌的抑菌活性均得到显著提高，同时CG的抗氧化性和抑菌活性明显优于CM。即与单糖进行美拉德反应制得的壳聚糖衍生物具有更好的生物活性。

Two kinds of chitosan oligosaccharide derivatives (CG and CM) were prepared by Maillard reaction of chitosan oligosaccharide (COS) with glucose and maltose, respectively. The molar ratio of amino group of COS and carbonyl group of glucose (maltose) was 1:1. The structural changes of CG and CM were confirmed by fourier transform infrared (FT-IR) spectra, and their molecular weights were determined by gel permeation chromatography (GPC) method. The antioxidant and antibacterial activities of COS, CG and CM were also assessed. The FT-IR spectra of CG and CM showed the characteristic absorption peaks of COS. The antioxidant activity and the antibacterial activity of CG and CM against Escherichia coli, Pseudomonas aeruginosa and Vibrio parahaemolyticus were higher than those of COS, and CG prepared by COS and monosaccharide-glucose showed better antioxidant and antibacterial activity.

National "Twelfth Five-Year" Plan for Science & Technology Support （2011BAD24B02）； Engineering Research Center of Shanghai Committee of Science and Technology (11DZ2280300)； Creative Activity Plan of Shanghai Ocean University (B-5106-11-0088)