中心组合设计优化超声辅助酶法提取玉米须皂甙工艺研究

Optimization of Ultrasonic-assisted Enzyme Extraction of Saponins by Central Composite Design from Corn Silk

摘要:

为研究玉米须皂甙的超声辅助酶提取工艺，应用中心组合试验设计，采用DPS软件进行二次多项式逐步回归分析，对提取工艺条件进行优化，确定玉米须皂甙的超声辅助酶提取的最佳条件：超声作用温度50 ℃、乙醇浓度90%、纤维素酶添加量1.62%、超声作用时间30 min，在此条件下玉米须皂甙提取率达2.24%。

Abstract:

To optimize the extraction process of saponins from corn silk, Central composite design combining quadratic polynomial stepwise regression analysis were applied to optimize extraction conditions. The optimal technological conditions can be concluded as follows: ultrasonic extraction temperature 50 ℃, ethanol concentration 90%, cellulase dosage 1.62% and ultrasonic extraction time 30 min. On these conditions, the yield of corn silk saponins was up to 2.24%.