正交实验优化脉红螺多糖的提取

Extraction of Polysaccharide from Rapana venosa by Orthogonal Test

摘要:

通过苯酚-硫酸法显色测定多糖的浓度，采用正交实验优化了脉红螺多糖的提取方法，为脉红螺的精深加工提供依据。在单因素分析的基础上进行正交试验，脉红螺多糖最佳提取条件为：提取温度50 ℃、料液比1:80（m/V）、提取时间30 min，此条件下，脉红螺多糖的得率为21.8%。

Abstract:

Extraction conditions for polysaccharide from Rapana venosa were optimized by orthogonal test. The effects of factors, including extraction time, solid to solvent ratio and extraction temperature were investigated using single factor analysis and orthogonal analysis. The results showed that the best conditions for extraction of polysaccharide from Rapana venosa were: extraction temperature 50 ℃; solid to solvent ratio 1:80 (m/V) and extraction time 30 min, under which the polysaccharide yield reached 21.8%.