除草剂扑草净胶体金免疫层析检测试纸条的研制

Development of a Colloidal Gold Immunochromatographic Strip for Rapid Detection of Herbicide Prometryn

摘要:

制备了扑草净胶体金免疫层析检测试纸条，建立了水样中扑草净的快速检测方法。采用柠檬酸三钠还原氯金酸法制备胶体金，对胶体金免疫层析方法的工作条件（NC膜的封闭条件、金标垫的处理方法）进行优化。最终建立的方法检出限为5 ng/mL，5 min内可以通过目测进行结果判断。河水样品不需要任何处理可以直接进行检测，检出限为5 ng/mL。本研究建立的快速免疫检测方法适用于水中扑草净残留的现场检测。

Abstract:

The colloidal gold immunochromatographic assay (CGIA) was applied in rapid detection of herbicide prometryn in water samples．Colloidal gold particles were prepared via trisodium citrate method. The CGIA conditions were optimized for high sensitivity. the detection limit of CGIA was 5 ng/mL and the detection process could be completed within 5 min. The river water samples were detected directly by the strip and the limit of detection was 5 ng/mL. The developed immunoassay in this study was a suitable tool for rapid screening residue of prometryn in water samples.