高静压处理对芸豆分离蛋白的聚集行为研究

Effect of High Pressure on Aggregation Behavior of Kidney Bean (Phaseolus Vulgaris L) Protein Isolate

摘要:

采用排阻色谱-多角度激光光散射-折光联用技术（SEC-MALLS-RI）研究高静压处理（HP）对芸豆分离蛋白分子量分布的影响规律，揭示HP导致KPI聚集-解离行为。根据Debye plot方法，计算出芸豆球蛋白的绝对分子量为161 kDa，200 MPa HP处理解离KPI的可溶性聚集物，而400和600 MPa HP处理诱导不溶性聚集物向可溶性聚集物转化，改善KPI的溶解度（PS）。

Abstract:

The influence of high pressure (HP) treatment (200-600 MPa) on molecular distribution of kidney bean (Phaseolus vulgaris L) protein isolate (KPI) was investigated by High-performance size-exclusion chromatography combined with multi-angle laser light scattering technique (HPSEC-MALLS), to explore the possible HP-induced protein association-dissociation behavior. The molecular weight (Mw) of phaseolin, the major component in KPI, calculated based on the Debye plot, was about 161 kDa. HP treatment at 200 MPa led to dissociation of soluble aggregates (at void volume), while HP treatment at 400 and 600 MPa led to dissociation of insoluble aggregates in KPI, thus improved PS.