环介导等温扩增技术检测花生过敏原

Application of Loop-mediated Isothermal Amplification Assay for Detection Peanut allergy

摘要:

花生引起的过敏已经越来越受到重视，因此对花生过敏原进行检测也变得越来越重要。目前对花生过敏原的检测大多数采用抗原抗体法或PCR方法，抗原抗体法耗时比较长，而PCR方法需要昂贵的仪器设备。本项目通过建立环介导等温扩增快速检测技术来检测食物中花生过敏原的基因，为食品中花生过敏原成分检测提供方便。该方法快速，简便，灵敏度高，可以很好的应用到现实检测中去，这个方法的建立具有很重大的意义，为食品的安全检测提供了很大的方便。

Abstract:

More and more attention is paid to peanut allergies, so the detection of peanut allergens is becoming increasingly important. In most case, antigen-antibody method and PCR method are mostly used in the detection of peanut allergen. However, antigen-antibody method takes longer, and the PCR method requires expensive equipment. In this report, the loop-mediated isothermal amplification (LAMP) method was developed to detect the gene of peanuts which is related to allergens producing. This method is rapid, simple and sensitive, which can provide convenience for peanut allergens detection. The establishment of the LAMP method has great significance and it provide a great convenience for food safety testing.