液相色谱法测定饮料中咖啡因、可可碱和茶碱

Determination of Caffeine, Theobromine and Theophylline in Drinks by HPLC

摘要:

采用高效液相色谱法同时测定饮料中的咖啡因、可可碱和茶碱。饮料样品经超声提取后，用高效液相色谱分离，二极管阵列检测器检测，建立标准工作曲线，以外标法定量。实验精密度为0.58%~1.85%，添加回收率范围为80.2%~96.4%。此方法快速，简便，准确，适用于饮料中咖啡因、可可碱和茶碱的测定。

Abstract:

A method for the determination of caffeine, theobromine and theophylline in drinks by HPLC was established. The drinks were extracted with water, separated by HPLC and then detected by photo-diode-array detector. Quantitative analysis was performed with external standard method. The relative standard deviations and recovery range were obtained of 0.58%~1.85% and 80.2%~96.4%, respectively. This simple method was suitable to detect the concentration of caffeine, theobromine and theophylline in drinks.