蒲公英抑菌提取液氯仿萃取部位高效液相色谱指纹图谱的研究

HPLC Fingerprint of Extracts of Taraxacum Bacteriostatic by Chloroform

摘要:

以咖啡酸作为对照物，构建蒲公英抑菌提取液氯仿萃取部位的高效液相色谱（HPLC）指纹图谱。本实验方法采用HPLC方法：Diamonsil C18（5 μm，4.6 mm×250 mm）色谱柱，流动相采用乙腈-水-10%乙酸溶液三相系统进行梯度洗脱，流速0.7 mL/min，检测波长323 nm，柱温37 ℃。指纹图谱得到12个共有色谱峰，并可根据这些色谱峰来控制蒲公英抑菌提取液的质量。

Abstract:

Caffeic acid was used as internal standard for the HPLC fingerprint establishment of chloroform extract from Taraxacum bacteriostatic. HPLC analysis was performed on a Diamonsil C18 column (5 μm, 4.6 mm×250 mm) with the three-phase system of acetonitrile-water-10% acetic acid solution as the mobile phase for gradient elution. The flow rate, detection wavelength and column temperature were 0.7 mL/min, 323 nm and 37 ℃, respectively. 12 Common peaks were showed in the fingerprint, which can be used to control the quantity of the Taraxacum bacteriostatic extraction.