ELISA法快速检测水产品中副溶血性弧菌

Application of Indirect ELISA Method for Rapid Detection of Vibrio parahaemolyticus

摘要:

以副溶血性弧菌抗原免疫新西兰大白兔获得特异性抗体，利用此抗体，进行间接竞争ELISA法实验，确定了抗原抗体最佳反应浓度和一抗稀释度分别为107 cfu/mL和1:4000，酶标二抗IgG-HRP最佳工作稀释度为1:1000。以此方法分别对人工染菌水产品及实际水产品进行检测，检测下限为104 cfu/mL，检测时间为8 h；而经8 h增菌后，其检测下限为103 cfu/mL。此法与常规方法检测结果完全一致，具有很高的实际应用价值。

Abstract:

The specific antibody was achieved by immune New Zealand white rabbit with V. parahaemolyticus antigen. Using this antibody, a indirect ELISA method for the detection of V. parahaemolyticus was developed with the best concentrations of the antibody, antigen and second antibody marked with HRP being of 107 cfu/mL, 1:4000, and 1:1000, respectively. The method can be used for the detection of V. parahaemolyticus in seafood with the detection limit and time of 104 cfu/mL and 8 h, respectively. However, the detection limit increased to 103 cfu/mL after an 8 h of bacteria multiplication. This rapid method had similar results with the conventional method, showing high practical application value.