槐花精油的化学成分及其抑菌活性的研究

Chemical Composition and Antimicrobial Activity of Essential Oil from Flos Sophorae Immaturus

摘要:

采用同时蒸馏萃取法提取槐花精油，用气相－质普联用技术（GC-MS）分析其化学成分；鉴定出43种化合物，占出峰总面积的98.49%；主要成分为十六酸（38.69%）、亚油酸甲酯（10.13%）、2-甲氧基-4-(2-丙烯基)-苯酚（6.71%）、亚麻酸（6.35%）、2-羟基-3-甲基-4H-吡喃-4-酮（3.49%）、8-十七碳烯（2.44%）、二苯砜（2.44%）、6,10,14-三甲基-2-十五酮（1.85%）、4-乙烯基-2-甲氧基苯酚（1.82%）等。体外抑菌实验显示该精油对金黄色葡萄球菌ATCC 6538、伤寒沙门氏菌CMCC50013、志贺氏痢疾杆菌CMCC51334、埃希氏大肠杆菌ATCC 8099均有抑制作用。

Abstract:

The chemical composition of the essential oil obtained from Flos Sophorae Immaturus by simultaneous distillation-extraction was analyzed by GC-MS. 43 Kinds of compounds (about 98.49% of the total peak areas) in the essential oil were identified and the major components were hexadecanoic acid(38.69%), 9,12-octadecadienoic acid methyl ester(10.13%), 2-methoxy-4-(2-propenyl)-phenol(6.71%), 9,12,15-Octadecatrienoic acid (6.35%), 2-hydroxy-3-methyl-4H-pyran-4-ketone(3.49%), 8-heptadecene(2.44%), 1,1'-sulfonylbis-benzene (2.44%), 6,10,14-trimethyl-2-pentadecanone(1.85%), 4-vinyl-2-methoxy-phenol(1.82%). In vitro antimicrobial experiments showed that the essential oil had antibacterial activities against Staphylococcus aureus ATCC 6538, Salmonella typhi CMCC 50013, Shigella dysenteriae CMCC 51334 and Escherichia coli ATCC 8099.