大孔树脂分离啤酒废酵母中谷胱甘肽的研究

Separation of Glutathione from Waste Beer Yeast by Macroporous Resin

摘要:

对大孔树脂D001分离啤酒废酵母中的还原型谷胱甘肽进行了研究。其最佳试验条件为：pH 4.5的0.02 mol/L磷酸缓冲液平衡，pH 7.5的0.02 mol/L磷酸缓冲液洗脱，洗脱流速为2.0 mL/min。在最佳分离条件下，大孔树脂D001分离GSH的平均收得率为20.03%，产品中GSH的含量为28.47%，产品颜色为白色。

Abstract:

Macroporous resin D001 was adopted for separation of GSH from waste beer yeast. The result indicated that the optimum concentration of phosphate buffer, pH values for adsorption and elution and the elution rate were 0.02 mol/L, 4.5, 7.5, and 2.9 mL/min, respectively. Under those conditions, the purity of the crude white GSH was 28.47% analyzed by HPLC, and the average yield was 20.03%.