高效液相色谱法测定蛋卷中的苋菜红和日落黄

Determination of Artificial Synthetic Pigments (Sunset Yellow and Amaranth) in Egg Roll by HPLC

摘要:

建立了测定蛋卷中合成色素日落黄和苋菜红的高效液相色谱分析方法。蛋卷中的合成色素经聚酰胺吸附提取，制成水溶液，过滤后用高效液相色谱仪测定。采用ODS柱分离，甲醇和乙酸铵溶液（0.02 mol/L）为流动相，测定蛋卷中苋菜红和日落黄，结果的相对标准偏差为1.5%~5.9%，实际蛋卷样品的回收率为71.5%~81.4%，检出限均为0.5 mg/kg。

Abstract:

A method for determination of two artificial synthetic pigments in egg roll (Sunset Yellow and Amaranth) by HPLC was established. the pigments in egg roll After purified was extracted by polyamide, dissolved in water, filtrated and then analyzed by HPLC with a ODS C18 column and the elution of methanol and ammonium acetate (0.02 mol/L). The detection limits, recovery and precision were 0.5 mg/kg, 71.5%~81.4%, and 1.5%~5.9%, respectively.