豆粕双酶复合水解工艺的研究

Enzymatic Hydrolysis of Soybean Residue by two kinds of Proteinase

摘要:

以豆粕为原料，利用多种蛋白酶对豆粕的蛋白质进行水解，挑选木瓜蛋白酶以及Flavourzyme两种蛋白酶进行复合酶解，得出酶解的最佳工艺参数为：木瓜蛋白酶的酶底比为600 U/g、Flavourzyme的酶底比为1200 U/g、Flavourzyme的作用时间为14.5 h、总时间为16 h、底物浓度为2%。水解后，总氮回收率达到55.73%。

Abstract:

The protein in soybean residue was hydrolyzed by various proteinase and two enzymes, papain and flaourzyme, were chosen for this purpose. The most suitable ratio of papain to substrate, ratio of flavourzyme to substrate, the reaction rime by flavourzyme, whole time of the hydrolysis and the substrate concentration were 600 U/g; 1200 U/g; 14.5 h, 16 h and 2%, respectively. After hydrolysis, the recovery of the total nitrogen reached 55.73%.