以枯草芽孢杆菌HJ-9(Xan-)为出发菌株，根据代谢工程原理，采用原生质体紫外诱变和微波诱变相结合的方法，定向筛选出了具有黄嘌呤缺陷、腺嘌呤脱氨酶阴性、磺胺胍抗性的腺苷生产菌HS-5，其摇瓶发酵产量由出发菌株的1.06 mg/mL，提高到3.15 mg/mL。

In this paper, Bacillus subtilis HJ-9(Xan-) was selected as the parent strain and treated by protoplast mutagenesis with ultraviolet and microwave, giving a adenosine-producing stain B.subtilis HS-5. The yield of the mutant increased from 1.06 mg/mL to 3.15 mg/mL.