由本实验室产胆固醇氧化酶的菌株类芽胞杆菌作为出发菌株，经过紫外+LiCl、紫外+光敏试剂（8—mop）物理化学诱变处理，最后得到一株突变株XUM-10，并对其进行发酵条件的优化，最终酶活为221.20 U/L，其酶活为出发菌株的2.5倍。

The mutation strain was obtained from Paenibacillus sp. X534 of our lab, and was mutagenized with Ultraviolet+LiCl and Ultraviolet+8-mop, giving XUM-10 strain. The fermentation conditions of XUM-10 strain were also studied. Results showed that the enzyme activity was 221.20U/L, which was 2.5 times higher than parent strain.