香芹酚抗氧化和抑制酪氨酸酶活性的研究

The Antioxidant and Antityrosinase Activities of Carvacrol

摘要:

采用清除DPPH、ABTS、羟自由基和超氧自由基，抑制脂质过氧化活性和抑制酪氨酸酶活性的方法，检测香芹酚的抗氧化活性和抑制酪氨酸酶活性。实验结果显示，香芹酚具有明显的抗氧化活性和抑制酪氨酸酶活性，呈浓度依赖性效应。在清除DPPH、ABTS、羟自由基、超氧自由基，抑制脂质过氧化活性和抑制酪氨酸酶活性试验中，香芹酚的体系终浓度IC50分别为100 μmol/L、18.18 μmol/L、16 μmol/L、16 μmol/L、12.5 μmol/L和6.67 μmol/L。作为阳性对照药物的山奈酚的体系终浓度IC50分别为80 μmol/L、9.09 μmol/L、12 μmol/L、12 μmol/L、8.33 μmol/L和3.33 μmol/L。香芹酚具有1个酚羟基，山奈酚具有3个酚羟基，分子结构的不同，导致山奈酚和香芹酚抗氧化活性和抑制酪氨酸酶活性不同。研究表明香芹酚具有明显的抗氧化活性和抑制酪氨酸酶活性，是值得进一步研究的抗氧化防衰老类候选药物、化妆品原料和食品添加剂。

Abstract:

The antioxidant and antityrosinase activities of carvacrol was investigated by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2‘-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical (?OH) and superoxide radicals, inhibiting lipid peroxidation and inhibiting tyrosinase activity. The experiment results showed that carvacrol had excellent antioxidant and antityrosinase activities in dose-dependent manner. In the experiments of scavenging DPPH, ABTS, ?OH and superoxide radicals, inhibiting lipid peroxidation and inhibiting tyrosinase activity, the IC50 of carvacrol were 100 μmol/L, 18.18 μmol/L, 16 μmol/L, 16 μmol/L, 12.5 μmol/L and 6.67 μmol/L, respectively. The IC50 of kaempferol used as the positive control drug were 80 μmol/L, 9.09 μmol/L, 12 μmol/L, 12 μmol/L, 8.33 μmol/L and 3.33 μmol/L, respectively. Carvacrol had one phenolic hydroxyl group, while kaempferol had three phenolic hydroxyls. The different molecular structures led to the different antioxidant and antityrosinase activities. In a word, carvacrol had good antioxidant and antityrosinase activities, worth being further researched as the new anti-aging candidate drug, cosmetic materials and food additives.