带鱼蛋白亚铁螯合肽抗贫血活性及安全性评价

Safety Evaluation and Anti-anemia Effect of Hairtail Protein Ferrous-chelating Peptide in Mice

摘要:

采用饲喂低铁饲料方法制备大鼠缺铁性贫血模型，灌胃带鱼蛋白亚铁螯合肽及应用急性毒性试验、Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验和体外哺乳动物细胞染色体畸变试验分别探究带鱼蛋白亚铁螯合肽抗贫血活性及安全性。结果显示：带鱼蛋白亚铁螯合肽能够提高贫血大鼠血红蛋白（Hb）含量和红细胞数（RBC）等外周血指标，显著促进大鼠贫血症状的改善。LD50=7454.80 mg/kg，属于实际无毒性；Ames试验中TA97、TA98、TA100和TA102的4个标准菌株皆为阴性结果；小鼠骨髓细胞微核试验、小鼠精子畸形试验和体外哺乳动物细胞染色体畸变试验组与阴性对照组比较差别无统计学意义。因此，Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验和体外哺乳动物细胞染色体畸变试验结果均为阴性。表明带鱼蛋白亚铁螯合肽具有抗贫血活性且食品安全性良好，可作为食品添加剂进一步开发利用。

Abstract:

A rat model of iron deficiency anemia was established by feeding rats an iron-deficient diet, and the anti-anemia effect and safety of hairtail protein ferrous-chelating peptide were studied by feeding the animals with hairtail protein ferrous-chelating peptide through gavage, and these effects were evaluated using the acute toxicity test, Ames test, mouse bone marrow micronucleus test, sperm abnormality test and chromosome aberration test in mammalian cells in vitro. The results showed that hairtail protein ferrous-chelating peptide increased the hemoglobin (Hb) content and the number of red blood cells (RBC) and other peripheral parameters, and significantly improved the symptoms of anemia in rats. The median lethal dose (LD50) was 7454.80 mg/kg, indicating actual non-toxicity. TA97, TA98, TA100, and TA102, four standard strains in Ames test, were all negative in the Ames test of the mice. Meanwhile, compared with the negative control group, the results of the mouse bone marrow micronucleus test, sperm abnormality test, and chromosome aberration test in mammalian cells in vitro showed no significant difference, suggesting that the three results were also all negative. Therefore, we concluded that hairtail protein ferrous-chelating peptide has an anti-anemia effect and good food safety, and can be further developed and utilized as a food additive.