不同容器发酵水豆豉乙醇提取物的抗氧化效果比较

Comparison of the Antioxidant Activities of Ethanol Extracts from Shuidouchi Samples Fermented using Different Vessels

摘要:

本研究对不同容器发酵水豆豉的抗氧化效果进行研究。首先对不同容器发酵水豆豉的理化特性进行了比较，玻璃容器发酵水豆豉（GVFS）的温度和酸度低于塑料容器发酵水豆豉（PVFS）和陶瓷容器发酵水豆豉（CVFS），水分含量和总菌数高于PVFS和CVFS。同时，对不同容器发酵水豆豉中的活性异黄酮含量也进行了分析，GVFS中的大豆黄素和金雀异黄素含量均高于PVFS和CVFS。体外抗氧化实验结果显示GVFS的DPPH自由基和?OH清除能力也高于其他两种水豆豉。进一步的动物实验中，GVFS可以提高D-半乳糖诱导氧化小鼠血清和肝组织中的SOD、GSH-Px水平和降低NO、MDA水平，且效果优于PVFS和CVFS。通过RT-PCR实验分析小鼠肝组织发现，GVFS能上调氧化小鼠的nNOS、eNOS、Mn-SOD、Gu/Zn-SOD、CAT mRNA表达和下调iNOS表达，使小鼠的这些表达最接近正常组小鼠。通过这些结果可以看出玻璃容器发酵的水豆豉比陶瓷和塑料容器发酵水豆豉具有更好的抗氧化效果，玻璃容器更适于功能性水豆豉的制作。

Abstract:

The antioxidant activities of Shuidouchi samples fermented in different vessels were determined in this study. First, the physicochemical properties of Shuidouchi samples fermented in different vessels were compared, and glass vessel-fermented Shuidouchi (GVFS) showed lower temperature and acidity and higher moisture contents and total bacteria counts than plastic vessel-fermented Shuidouchi (PVFS) and ceramic vessel-fermented Shuidouchi (CVFS). The active isoflavone contents in Shuidouchi samples fermented in different vessels were measured, and the daidzein and genistein contents in GVFS were higher than those in PVFS and CVFS. The in vitro antioxidant experiments showed that the 2,2-diphenyl-1-picrylhydrazyl (DPPH?) free radical and hydroxyl (?OH) radical scavenging capacities of GVFS were also higher than those of PVFS and CVFS. Animal experiments showed that GVFS increased the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and decreased the levels of nitric oxide (NO) and malondialdehyde (MDA) in serum and liver of mice with D-galactose induced oxidative stress, and these effects of GVFS were better than those of PVFS and CVFS. Reverse transcription polymerase chain reaction (RT-PCR) showed that GVFS upregulated the expression of nNOS, eNOS, Mn-SOD, Gu/Zn-SOD, and CAT mRNA and downregulated iNOS expression in the liver of mice subjected to oxidative stress, indicating that these expression levels were close to those in normal mice. Based on these results, GVFS had higher antioxidant activity than PVFS and CVFS, and a glass vessel is suitable for Shuidouchi production.