拐枣多糖对环磷酰胺诱导免疫低下小鼠免疫功能的影响

Effect of the Polysaccharides from Hovenia acerba Lindl. on Immune Activity in Cyclophosphamide-Induced Immunodepressed Mice

摘要:

本文旨在研究拐枣多糖（Hovenia acerba Lindl.polysaccharide,HAP）对环磷酰胺(CTX)诱导免疫低下小鼠免疫功能的影响。除空白对照组用生理盐水（NS，0.1 mL/10 g）灌胃外，其它各组小鼠于给药的第1、3、5、7、9 d腹腔注射CTX (30 mg /kg)造模。将建模成功体重相近的小鼠随机分为模型组、HAP高、中、低剂量(400、200、100 mg /kg bw)处理组，另设正常对照组。连续灌胃给药 28 d，研究HAP对模型小鼠胸腺指数、脾脏指数、脾淋巴细胞增殖、廓清指数、吞噬指数、半数溶血值、血清中IL-2、IL-4、IFN-γ 生成水平和足趾厚度差等指标的影响。结果显示，HAP各剂量均能显著提高模型小鼠脾脏指数、脾淋巴细胞增殖作用、廓清指数、吞噬指数、半数溶血值及足趾厚度差（p<0.05）；HAP低、中、高剂量促使IL-2、IL-4、IFN-γ生成水平均明显提高（P<0.05）。研究结果表明，拐枣多糖对环磷酰胺致免疫低下小鼠的非特异性免疫和特异性免疫具有增强作用。

Abstract:

The impact of Hovenia acerba Lindl. (HAP) polysaccharides on immune activity in cyclophosphamide (CTX)-induced immunodepressed mice was studied. Except for the control group, mouse models for all other groups were constructed by intraperitoneal injection with CTX (30 mg/kg) on days 1, 3, 5, 7, and 9, respectively, and the control group was administrated normal saline (NS, 0.1 mL/10 g) by gavage．After the mouse model was established, mice with similar weight were randomly divided into five groups: normal control group, immunodepression model group, and HAP-treated groups (low-, medium-, and high-dose). The intragastric administration of HAP was performed continuously for four weeks, and the effects of HAP on the thymus and spleen indices, spleen lymphocyte proliferation, clearance index, phagocytic index, the concentration causing 50% hemolysis (HC50), the levels of serum interleukin (IL)-2, IL-4, and interferon gamma (IFN-γ), and the difference in toe thickness in CTX-induced immunodepressed mice were assessed. Compared to the CTX group, the spleen index, clearance index, phagocytic index, spleen lymphocyte proliferation, HC50, and the difference in toe thickness were enhanced significantly (p ＜ 0.05) in the HAP groups (with all doses). Levels of serum IL-2, IL-4, and IFN-γ were significantly increased in the low-, medium- and high-dose HAP groups (p ＜ 0.05) in CTX-induced immunodepressed mice. These results suggested that HAP could improve non-specific and specific immunological activities in immunodepressed mice．