莲房原花青素对极低频电磁场致免疫功能损伤的预防作用

Preventive Effects of Lotus Seed Pod Procyanidins on Extremely Low Frequency Electromagnetic Exposure-induced Immune Function Injury

摘要:

为了探讨莲房原花青素（LSPCs）对极低频电磁场（ELF-EMF）致小鼠免疫功能损伤的影响，通过半体外法研究LSPCs对脾脏淋巴细胞增殖、NK细胞活性以及血浆和肝脏中细胞因子IL-4和INF-γ的影响，初步确定其免疫调节活性。MTT法检测T、B淋巴细胞增殖、乳酸脱氢酶法检测 NK 细胞活性、ELASA试剂盒检测IL-4和INF-γ浓度。结果表明：与正常组相比，ELF-EMF辐照后胸腺和脾脏指数显著降低（p<0.05，p<0.01），脾脏T、B淋巴细胞增殖率和NK细胞活性显著降低（p <0.01）、IL-4和INF-γ浓度明显降低；而经LSPCs预处理后，与ELF-EMF组相比上述指标均有所改善，其中90 mg/kg LSPCs组，显著提高了胸腺和脾脏指数（p <0.05，p <0.01），能显著地刺激了T、B淋巴细胞的增殖、提高了NK细胞活性（p <0.01），并且促进了血浆和肝脏分泌IL-4和INF-γ。由此显示LSPCs可以有效地预防ELF-EMF致小鼠免疫功能损伤，调节免疫活性。

Abstract:

To explore the effects of lotus seed pod procyanidins (LSPCs) on extremely low frequency electromagnetic field (ELF-EMF) exposure-induced immune function injury in male mice, a semi-in vitro method was used to study the effect of LSPCs on splenic lymphocyte proliferation, natural killer (NK) cell activity, interleukin 4 (IL-4), and interferon gamma (IFN-γ) in the plasma and liver. In addition, the immunomodulatory activity of LSPCs was preliminarily determined. The proliferation of T and B lymphocytes was measured by a [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] tetrazolium (MTT) reduction assay. NK cell activity was determined by a lactate dehydrogenase (LDH) release assay, and the concentrations of IL-4 and IFN-γ in plasma and liver were measured by an enzyme-linked immunosorbent assay (ELISA) kit. Our experiments indicated that after ELF-EMF radiation, the spleen and thymus indices were significantly decreased (p< 0.05, p < 0.01, respectively) compared to those of the control. MTT and LDH release assays showed that the proliferation of T and B lymphocytes and NK-cell activity decreased significantly in the ELF-EMF group (p < 0.01). The concentration of IL-4 and IFN-γ decreased significantly in the plasma and liver. However, pretreatment with LSPCs improved the above indicators compared to those of the ELF-EMF group. Especially for the ELF-EMF+LSPCs90 group, the spleen and thymus indices increased significantly (p < 0.05, p < 0.01, respectively), the proliferation of T and B lymphocytes was significantly stimulated, and NK cell activity and secretion of IL-4 and IFN-γ in the plasma and liver were promoted. In conclusion, LSPCs can effectively prevent the ELF-EMF-induced immune injury and regulate immune activity.