环介导等温扩增技术快速检测奶粉中的单增李斯特菌

Rapid Detection of Listeria monocytogenes in Milk Powder by Loop-mediated Isothermal Amplification

摘要:

环介导等温扩增技术(Loop-mediated isothermal amplification, LAMP)是一种在等温条件下特异、灵敏、快速的新型基因扩增技术。试验以单增李斯特菌为研究对象，根据其特有的hlyA 基因设计了一套特异性引物，进行LAMP扩增。结果表明，LAMP检测单增李斯特菌的灵敏度为2.45×101 cfu/mL，其对人工感染奶粉样品的检出限为7.32×101 cfu/mL。对其它食品病原菌进行检测，结果均未出现目的条带，特异性强。表明LAMP法适合于食品中污染单增李斯特菌的快速检测。

Abstract:

Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification technology with high specificity, efficiency and rapidity under isothermal conditions. In this research, a set of specific LAMP primers were designed to amplify the specific hlyA gene of Listeria monocytogenes by LAMP technology. The results showed that the detection sensitivity and detection limit of the LAMP method for purely cultured Listeria monocytogenes were 2.45×101 cfu/mL and 7.32×101cfu/mL, respectively. For other food pathogens, the target bands were not found . This method was suitable for rapid detection of Listeria monocytogenes in contaminated foods.