Volume 37,Issue 4,2021 Table of Contents

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  • 1  Gongcheng Oil Tea Attenuates Oxidative Stress in D-galactose-Induced Aging Mice
    NIU Jia-wei MO Ling WANG Zi-zeng LI Jing-jing
    2021, 37(4):1-6. DOI: 10.13982/j.mfst.1673-9078.2021.4.0913
    [Abstract](315) [HTML](153) [PDF 1.77 M](836)
    Abstract:
    Effect of Gongcheng oil tea on oxidative stress in D-galactose-induced aging mice was investigated. Healthy KM mice of reproductive age were randomly divided into 4 groups: normal control, aging model, Vc groups (100 mg/kg) and Gongcheng oil tea groups (750 mg/kg). The enzyme activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (T-SOD), as well as the total antioxidant capacity (T-AOC) and malondialdehyde (MDA) level in serum, liver and kidney tissue of mice were determined. The pathological changes of liver and kidney tissues were observed. Gongcheng oil tea could significantly improve the liver and kidney indexes of mice that decreased by D-galactose (p<0.05). Compared with those of the aging group, the liver and kidney indexes of the oil tea group increased by 11.87% and 25.38% respectively. Pretreatment with Gongcheng oil tea, the enzyme activities of T-SOD and GSH-Px, as well as T-AOC levels in serum, liver and kidney tissue of mice increased significantly, while MDA production in serum, liver and kidney tissue of mice decreased significantly (p<0.05). Compared with the senescence group, GSH-PX, T-SOD and T-AOC in the liver of oil tea group increased by 62.98%, 98.35% and 714.63%, respectively (p<0.05), and MDA decreased by 65.42% (p<0.05). The activities of GSH-Px, T-SOD and T-AOC in kidney increased by 119.66%, 182.81% and 155.88% (p<0.05), respectively, while MDA decreased by 74.61% (p<0.05). Pathological observation showed that Gongcheng oil tea could alleviate liver and kidney damage during aging. The results showed that Gongcheng oil tea could inhibite aging-induced oxidative stress-related liver and kidney injuries in mice by attenuating oxidative stress.
    2  Study on the Effect of Jupi Decoction on the Structure of Intestinal Flora Based on SHIME
    LUO Yu-shuang WU Jing-yi LIU Shi-feng KANG Xin-cong WANG Lei ZHANG Zhi-xu LIU Dong-bo
    2021, 37(4):7-15. DOI: 10.13982/j.mfst.1673-9078.2021.4.0782
    [Abstract](378) [HTML](265) [PDF 2.59 M](729)
    Abstract:
    This study was based on the simulator of the human intestinal microbial ecosystem (SHIME), and human intestinal microorganisms were introduced to simulate in vitro the human microbial ecology. The effects of jupi decoction on intestinal flora were examined by 16S rRNA high-throughput sequencing technology, and the effects of jupi decoction on the metabolites of intestinal microflora, short-chain fatty acids (SCFAs), were investigated by gas chromatography. The results showed that after the intervention of jupi decoction, the relative abundance of Veronica decreased by 33.65% during the intervention period and by 92.78% during the maintenance period. The relative abundance of Klebsiella decreased by 63.60% and 67.82%, respectively, during the intervention and maintenance periods, while that of Macromonas increased by 27.5% and 62.61% during the intervention and maintenance periods, respectively. The content of acetic acid among the short chain fatty acids decreased first and then increased (by 3.21% in general), while the content of propanoic acid decreased by 45.43% (p<0.01). The contents of isobutyric acid, butyric acid, isovaleric acid and valeric acid increased by 52.94%, 40.86%, 48.94% and 80.00%, respectively. The intervention with jupi decoction can change the diversity of intestinal microflora, regulate the level of microbial abundance, exhibit inhibitory effects on the growth of harmful intestinal microorganisms such as Veronica and Klebsiella, and promote the production of intestinal microbial metabolites, short chain fatty acids, which improves effectively the structure of human intestinal flora.
    3  Antioxidant and Anti-proliferative Activities of Polyphenols from Sterculia nobilis Smith Huskon Human Lung Cancer Cells A549
    LI Yuan ZHAO Zhen-gang
    2021, 37(4):16-24. DOI: 10.13982/j.mfst.1673-9078.2021.4.0426
    [Abstract](306) [HTML](301) [PDF 850.23 K](703)
    Abstract:
    In this study, the free and bound polyphenols were extracted from Sterculia nobilis Smith husk using Sterculia nobilis Smith husk as the raw materials. Then, the free polyphenols were extracted with organic solvents of different polaritiesto yield a four phase-extract, namely n-hexane phase (HF), ethyl acetate phase (EAF), n-butanol phase (BF) and aqueous phase (AqF). The total phenolic contentand total flavonoid content of the free polyphenol extract, bound polyphenol extract and four-phase extract were measured. The results showed that the free polyphenolextract had higher total phenolic content and total flavonoid content than the bound polyphenol extract. Among the four phases in the four-phase extract, the EAF had the highest phenolic and flavonoid contents.DPPH free radical scavenging ability, ABTS free radical scavenging ability and reducing power assays were used to evaluate comprehensively the antioxidant activities of the bound polyphenol extract and four-phase extract. EAF showed a higher antioxidant activity, with the EC50 values for DPPH and ABTS free radicals being 2.08 mg/mL and 29.28 mg/mL. The reducing power of EAF reached 0.81 at a concentration at 10 mg/mL. Eleven phenolic compounds (epicatechin, vanillic acid, isoorientin, ferulic acid, rutin, quercitrin, myricetin, trilobatin, quercetin and kaempferol) were identified in EAF by HPLC. In addition, EAF had a dose-dependent inhibitory effect on the proliferation of human lung cancer cell A549 with an EC50 value of 85.70 μg/mL. The results indicate that the polyphenol extract from Sterculia nobilis Smith husk has significant antioxidant activity and anti-proliferative activity towards A549 cells, which is helpful for the comprehensive utilization of Sterculia nobilis Smith husk and the development of new functional agents.
    4  Analysis of the Effects of Angelica sinensis Ultrafine Powder on the Diversity of Intestinal Flora in Mice Based on 16S rRNA Technology
    BAI Xue-song LIU He SONG Yan LV Guang-fu LIN He LIN Zhe LI Yin-qing
    2021, 37(4):25-32. DOI: 10.13982/j.mfst.1673-9078.2021.4.0708
    [Abstract](345) [HTML](223) [PDF 4.13 M](837)
    Abstract:
    The effects of different doses of ultrafine powder on the diversity of intestinal flora and physiological structure of the intestinal tract in mice were studied. C57BL/6 mice were divided into high-dose ultrafine powder group (2.0 g/kg), medium-dose group (1.0 g/kg), low-dose group (0.5 g/kg) and traditional decoction piece group (2.0 g/kg), the traditional powder group (2.0 g/kg) and the blank group, and subject to intragastric administration for 15 days. The body weights were recorded. On the 15th day of gavage, the colon contents were taken out aseptically, the V3+V4 region of the 16s rRNA gene of the intestinal flora was amplified, and the beta diversity and alpha diversity were analyzed by high-throughput sequencing. The compositions of the species at the phylum level and genus level were analyzed, and the pathological changes of the gastrointestinal tract was examined by HE staining. The results showed that the body weight of C57BL/6 mice grew normally. The high-dose of Angelica sinensis ultrafine powder decoction pieces significantly increased the content of Verrucomicrobia (p<0.05) and decreased the content of Actinobacteria (p<0.01). In addition, in the medium-dose group of Angelica sinensis ultrafine powder decoction pieces, the content of Bacteroidetes decreased the most with the most significant increase of Firmicutes. At the genus level, the medium-dose increased the most significantly the content of Faecalibaculum (p<0.05). Therefore, the medium dose of ultrafine powder was the most suitable experimental dose. There was no pathological change in the gastrointestinal tract of mice in each treatment group. This experiment can provide a theoretical basis for the scientific dosage and safety of Angelica sinensis ultrafine powder for human health care or treatment, and offer technical support for the effectiveness of the intestinal flora.
    5  Effects of Epigallocatechin-3-gallate on Nonalcoholic Fatty Liver Disease in Mice
    SHEN Jing SHU Heng SHI Meng-qiong ZHANG Yuan-yuan ZHU Li-jin LEI Qian-kun ZHANG Ji-hong HE Hai-bo ZOU Kun
    2021, 37(4):33-43. DOI: 10.13982/j.mfst.1673-9078.2021.4.0848
    [Abstract](245) [HTML](262) [PDF 4.80 M](729)
    Abstract:
    The protective effects of epigallocatechin gallate (EGCG) on nonalcoholic fatty liver disease (NAFLD) in mice were investigated. The NAFLD ApoE-/- mice model was established by feeding high fat food. Then, the effects of EGCG on glucose and lipid metabolism, fatty acid oxidation, oxidative stress level, liver tissue structure and the gene expressions of AMPK/SIRT1/SREBP-1c/PPARγ signaling pathway in NAFLD mice were analyzed. The results indicated that after EGCG treatment, the pathological changes of liver tissue were evidently ameliorated; compared with the model group, the body weight, liver mass and liver mass index of mice were significantly reduced to 110.98%, 115.01%, 115.64%, 136.48% and 104.20%, 118.70% respectively (p<0.01, respectively). The levels of glucose and lipid metabolism, fatty acid oxidation and oxidative stress in blood and liver tissues were markedly improved by 11.85%~86.06% compared with model group (p<0.05 or p<0.01, respectively). Further study indicated that the AMPKα, SIRT1 mRNA and p-AMPKα, SIRT1 protein expressions in liver tissue were obviously elevated by 13.21%~75.82%. In contrast, the FASN、ACC-1、SREBP-1c、SCD-1、PPARγ mRNA and FASN、p-ACC-1、p-SREBP-1c、SCD-1、PPARγ protein expressions in liver tissue were dramatically reduced by 19.59%~92.07%, the p-AMPKα/AMPKα、p-ACC-1/ACC-1 and p-SREBP-1c/SREBP-1c ratios significantly ameliorated by 39.20%~93.07% compared with model group (p<0.05 or p<0.01, respectively). The results demonstrated that EGCG could ameliorate glucose and lipid metabolism, fatty acid oxidation and oxidative stress in NAFLD mice by regulating there lated gene expressions of AMPK/SIRT1/SREBP-1c/PPARγ signaling pathway.
    6  Inhibitory Effect of Selenium-rich Protein in Green Tea on HepG2 Cells of Liver Cancer
    YAO Xing-mei GUO Dan YAO Shuai
    2021, 37(4):44-49. DOI: 10.13982/j.mfst.1673-9078.2021.4.0825
    [Abstract](306) [HTML](257) [PDF 875.57 K](631)
    Abstract:
    The inhibitory effect of the selenium-rich proteins in green tea on HepG2 cells of live cancer was analyzed in this study. HepG2 cells in the logarithmic growth stage were incubated in the DEEM medium and used as a control group. The selenium-rich proteins of green tea at concentrations of 0.11 g/L, 0.18 g/L, 0.25 g/L and 0.5 g/L, respectively, were used as the experimental groups. The DMEM medium supplemented with dimethyl sulfoxide (DMSO) was used as the reference group. The inhibition rate was calculated by the optical density value, the apoptotic rate was detected by flow cytometry, and apoptosis-related factors were detected experimentally. The results showed that the inhibitory rate for the HepG2 cells of liver cancer increased with time and the concentration of selenium-rich proteins in green tea, but the inhibition rate for HepG2 cells for the control group was always zero. The apoptotic rate for the HepG2 cells of the control group was 6.17%, and the maximum apoptotic rate reached the maximum (30.60%) when the concentration of selenium-rich proteins of the green tea was 0.5 g/L. The expression level of apoptosis factors of the experimental groups showed an overall downward trend, the Bcl-2 and Bax ratio was lower than 1, indicating that the higher the concentration of the selenium-rich proteins of green tea, the longer the incubation time, the stronger the ability to inhibit HepG2 tumor cells, selenium-rich proteins. Therefore, green tea’s selenium-rich proteins can significantly decrease the number of S-phase cells, which indicates that the green tea selenium-rich proteins can inhibit HepG2 cells of liver cancer.
    7  Short-term Oral Administration of Inulin can Improve the Fatigue Resistance of Mice
    CHI Ming ZHAO Yu-fan GAO Feng XIANG Cen CHEN Kai-yang TENG Yu-ou ZHANG Dong
    2021, 37(4):50-55. DOI: 10.13982/j.mfst.1673-9078.2021.4.0661
    [Abstract](258) [HTML](137) [PDF 1.65 M](734)
    Abstract:
    In this study, the effects of inulin on improving mouse's anti-fatigue and intestinal flora-regulating effects were investigated. The mice were administered orally at low (0.4 mg/kg) and high (2 mg/kg) doses of inulin for 45 days. Thirty minutes after the last administration, the time of weight-loaded swimming was recorded, and the serum creatine kinase (CK) activity, the blood urea nitrogen (BUN) and lactic acid (LA) contents and the fecal flora composition of mice were measured after the forced swimming. The results showed that the weight-loaded swimming times of the low-dose and high-dose groups increased by 45.62% and 90.52%, respectively, compared with the blank group (p<0.05). After strenuous exercise, compared with the blank group, the CK activities of the low-dose group and high-dose group decreased to 17.07% and 53.65% (p<0.05), respectively, with their BUN contents decreasing by 68.70% and 81.28% (p<0.05), respectively, and LA contents decreasing to 16.29% and 41.85% (p<0.05), respectively. In terms of regulating the gut microbiota, compared with the control group, the harmful bacteria (Proteobacteria) in the intestinaltract were down-regulated in the administration group. The experimental results showed that inulin has a significant anti-fatigue effect and regulatory effect on intestinal flora abundance.
    8  Metabolomics-based Analysis of the Importance of Slr0643 Protein in Regulating Synechocystis sp. PCC6803 to Acclimate to Glucose Mixotrophic Conditions
    XU Bai-xue CHEN Gu LIU Cheng-li ZHOU Jian
    2021, 37(4):56-63. DOI: 10.13982/j.mfst.1673-9078.2021.4.0359
    [Abstract](270) [HTML](287) [PDF 8.39 M](785)
    Abstract:
    In this study, a wild-type strain of Synechocystis sp. PCC6803 and a Slr0643 knockout mutant (Δslr0643) strain were used. The mechanism underlying the regulatory role of Slr0643 protein in the acclimation of Synechocystis sp. PCC6803 to glucose mixotrophic conditions was investigated through physiological experiments and gas chromatography-mass spectrometry (GC-MS)-based plant metabolomic analysis. Under mixotrophic conditions with 2.5 mM glucose, the growth rate of the wild-type was significantly higher than that under autotrophic condition, while the growth rate of Δslr0643 strain was significantly lower than that under autotrophic condition. After four days of mixotrophic growth with glucose, the OD730 value of Δslr0643 mutant was only 47.25% of that for the wild type, and the glucose utilization rate of Δslr0643 mutant was consistently lower than that the wild-type strain. Through the identification of the differential metabolites of the Δslr0643 mutant strain and wild-type strain over the mixotrophic cultivation with 2.5 mM glucose, 11 significantly different metabolites were found, with the contents of 9 differential metabolites in the wild-type strain increasing significantly during mixotrophic cultivation. Through the enrichment analysis of the metabolic pathways involved in the different metabolites of the mutant strain relative to the wild -type strain at each culture time point, it was found that the differential metabolites were mainly enriched in 8 metabolic pathways, the tricarboxylic acid cycle, pyruvate metabolism, glutamate metabolism, suggesting that Slr0643 protein was involved in regulating these metabolic pathways to make Synechocystis sp. PCC6803 adapt to glucose mixotrophic conditions. This study showed that the deletion of slr0643 damaged multiple metabolic pathways in cells, thereby making the cells unable to utilize glucose normally, and Slr0643 plays an important role in the acclimation of Synechocystis sp. PCC6803 to glucose mixotrophic conditions.
    9  Optimization of Yeast and Fermentation Process for Pomegranate Wine with Low Higher Alcohol
    ZHU Yu-zhang WANG Jun-ren LI Xi-zi ZHANG Cui-ying
    2021, 37(4):64-71. DOI: 10.13982/j.mfst.1673-9078.2021.4.0921
    [Abstract](282) [HTML](226) [PDF 574.79 K](589)
    Abstract:
    In order to investigate the effects of yeast species and fermentation conditions on the higher alcohol content of pomegranate wine, pomegranate juice was used as raw material, and two different fermentation processes was adopted to screen Saccharomyces cereviscerae. The brewing conditions were optimized to produce pomegranate wine with low yield of higher alcohol. Through the determination of physical and chemical properties of pomegranate wine, the higher alcohol content was considered as the main index to screen out the best yeast and optimal conditions. Results show that WY-T21 yeast could produce the lowest content of higher alcohol, and the total yield of higher alcohol was 272.26 mg/L, which was 27.74% lower than that of WY-1 yeast. The higher alcohol content was used as the main index and other physical and chemical properties analysis was also considered. It can be concluded that temperature had the greatest influence on the fermentation speed, while the dipping time had the greatest influence on the ethanol content. The optimum fermentation conditions for low yield of higher alcohol pomegranate wine were as follow: immersion time of 6 days, addition of sulfur dioxide of 80 mg/L, and fermentation temperature of 20 ℃. Under this optimal fermentation process, the total content of advanced alcohol was 245.35 mg/L, which was 9.88% lower than that of the control. In conclusion, the research and development of low content of higher alcohol pomegranate wine could provide new research ideas for the development of higher quality wine.
    10  Modification of Strong Promoter PCAT1 of Pichia pastoris and Analysis of Transcription Factor Binding Sites
    NONG Lu-yuan LIANG Shu-li
    2021, 37(4):72-78. DOI: 10.13982/j.mfst.1673-9078.2021.4.0324
    [Abstract](467) [HTML](348) [PDF 7.17 M](1030)
    Abstract:
    In this study, the methanol-inducible strong promoter PCAT1 of Pichia pastoris was modified by random mutation, characterized by green fluorescent protein. After high-throughput screening, a PCAT1 promoter library with an activity range of 25.57%- 138.12% was obtained. It was found that the PCAT1 variant 6-39 whose activity increased by 38.12% was mutated from A to G at the -118 bp in the 5 '-3'direction.The online prediction software for transcription factor binding sites, MatInspector, was used to analyze the change of transcription factor binding sites of the PCAT1 variants. Compared to wild-type PCAT1, the binding site of F$YMCM of the PCAT1 variant was reduced, and the binding sites of F$CSRE and F$YGAL increased. Then, the transcription factor binding sites of 16 promoters of the genes involved in methanol utilization pathway of Pichia pastoris were analyzed by MatInspector. It was found that the binding sites of the transcription factor, F$CSRE, were widely distributed in the methanol-inducible promoters, whilst the binding sites of F$YGAL were extensively distributed only in the constitutive promoter. Thus, the increase of the binding sites of F$CSRE is most likely to increase the methanol-inducing activity of PCAT1, which provides a reference for the analysis of the key sites of the promoters of the genes for the methanol utilization pathway of Pichia pastoris.
    11  Degradation Effect of Three Photosensitizers-Mediated Photodynamic Technology on Eight Polycyclic Aromatic Hydrocarbons in Water
    LU Na LI Zhao-jie WANG Zhi-guang TANG Qing-juan XUE Chang-hu XUE Yong XU Jie
    2021, 37(4):79-86. DOI: 10.13982/j.mfst.1673-9078.2021.4.0887
    [Abstract](299) [HTML](180) [PDF 1.56 M](723)
    Abstract:
    This study aims to explore the degradation effect of different photosensitizers (curcumin, riboflavin, hypericin)-mediated photodynamic technology on polycyclic aromatic hydrocarbons (PAHs) in water. 8 PAHs mixed aqueous solution was taken as the research object. Under the condition of 10 μM photosensitizer concentration and 15min illumination time, the mixture was treated by photodynamic technology (PDT) and the PAHs were determined by synchronous fluorescence method. The results showed that the degradation of PAHs by curcumin-mediated photodynamic was significantly better than that of riboflavin and hypericin-mediated photodynamic groups. After photodynamic treatment of curcumin, 8 kinds of PAHs in the mixture were degraded to some extent. The degradation rates of fluoranthene and phenanthrene were 77.07% and 78.31 %, respectively. The degradation rates of benzo [k] fluoranthene and chrysene were 80.10% and 85.95%, respectively. The degradation rates of benzo [a] pyrene, naphthalene, benzo [a] anthracene and anthracene were more than 95%. Our research showed that compared with the other two photosensitizers, curcumin-mediated photodynamic had a significant effect on the degradation of 8 PAHs in aqueous solution, which provides a theoretical basis for the promotion and application of this technology in the pollution control of living and aquaculture water.
    12  Analysis and Evaluation of Different Farming Modes and Nutrient Composition of Wild Crawfish (Procambarus clarkia) Muscle
    CHENG Xiao-fei SONG Rui XIANG Jin LIU Li TIAN Xing WANG Dong-wu LIU Ming-qiu XIE Zhong-gui WANG Jin-long
    2021, 37(4):87-95. DOI: 10.13982/j.mfst.1673-9078.2021.4.0823
    [Abstract](347) [HTML](158) [PDF 441.71 K](696)
    Abstract:
    To evaluate comprehensively the nutritional value of the muscle from pond-reared Procambarus clarkia, Procambarus clarkia reared in paddy field, and wild Procambarus clarkia, the compositions and contents of their muscles were determined. The results showed that the crude protein content of muscle decreased in this order: paddy field group (84.42%)>pond group (83.59%)>wild group (82.81%) (p>0.05). The crude fat content of the muscle was in this order: paddy field group (2.96%)> pond group (2.22%)>wild group (1.48%) (p<0.05). The values of TAA, ∑EAA, ∑EAA/∑TAA, ∑EAA/∑NEAA and ∑DAA/∑TAA groups followed this order: pond group> rice field group> wild group (p>0.05). According to AAS, the first limiting amino acids of the pond-reared, paddy field-rear and wild Procambarus clarkii were Thr, Val and Val, respectively. The second limiting amino acids were all Met+Cys. According to CS, the first limiting amino acids were all Met+Cys,and the second limiting amino acids were Val, Thr and Val, respectively. There were 13, 14 and 15 kinds of fatty acids detected in the muscle of the pond-reared, paddy field-rear and wild Procambarus clarkii, of which ∑SFA was 48.06%, 44.03% and 39.95%, respectively(p<0.05); ∑MUFA was 35.48%, 31.03% and25.76%, respectively(p< 0.05); ∑PUFA was 16.46%, 24.93% and32.30%, respectively(p< 0.05).The n-3/n-6 ratios were in this descending order: wild group (0.18)
    13  Antifungal Activities of Extracts and Chemical Constituents Analysis of N-butanol Extract from Phellodendron chinense
    MA Xin GHENG Yan MA Rong-li
    2021, 37(4):96-101. DOI: DOI: 10.13982/j.mfst.1673-9078.2021.4.1060
    [Abstract](393) [HTML](167) [PDF 511.71 K](711)
    Abstract:
    The antifungal activity and chemical components of the extracts of Phellodendron chinense with the strongest antifungal effect were investigated to provide the foundation for the development of new botanical fungicides. The extract of Phellodendron chinense was prepared by ultrasonic-assisted ethanol extraction, and then extracted with equal volumes of petroleum ether, chloroform, ethyl acetate, and n-butanol, respectively. The antifungal activity of each extract was determined by means of the mycelium growth rate method. The chemical components of the extract with the strongest antifungal effect were analyzed by GC-MS. The results indicated that n-butanol extract had the strongest inhibitory effect, with an antifungal rate of 84.46%, a minimum inhibitory concentration (MIC) of 20 mg/mL, and a half maximal inhibitory concentration (EC50) value of 6.480 mg/mL, respectively. GC-MS analysis results showed that 26 compounds were separated and identified from n-butanol extract, mainly alkaloids, organic acids, glycosides and amides. The component with the highest relative content was 5-(2-Thienyl)-4-pyrimidinamine, accounting for 52.85%. The antifungal active ingredients of extracts of Phellodendron chinense branches and barks were mainly present in the n-butanol part, and were dominated by the alkaloids compound. It was supposed that the alkaloids compound was the main antifungal active ingredient of Phellodendron chinense branches and barks in inhibition of fungal diseases of plants, providing the foundation and reference basis for development and application of botanical fungicides.
    14  Preparation of Antimicrobial Peptide Piscidin 1 PG/ Polylactic Acid Electrospun Nanofiber Membrane and Its Preservation Effect on Sea Bass
    XU Yu-chen LI Jian-rong LI Ting-ting XIE Jing LAO Min-jun WANG Hong GUO Xiao-hua
    2021, 37(4):102-109. DOI: 10.13982/j.mfst.1673-9078.2021.4.0662
    [Abstract](262) [HTML](234) [PDF 893.02 K](621)
    Abstract:
    In this study, Piscidin 1 PG/polylactic acid (Pis-1 PG/PLA) nanofiber membrane was prepared by an electrospinning method. The morphology, tensile strength, structure, thermal properties and mechanical properties of the nanofiber membrane as well as the growth of Shewanella putrefaciens were investigated. Finally, the sea bass meat was wrapped with Pis-1 PG/PLA fiber membrane, and the preservation effect of the membrane was studied through measuring the total number of colonies, pH and total volatile basic nitrogen (TVB-N) during 15 days of cold storage at 4 ℃. The results showed that Pis-1 PG/PLA nanofibers were linear with a smooth and disordered structure; the addition of Pis-1 PG decreased the average diameter of Pis-1 PG/PLA fiber membrane to 225 nm, increased the tensile strength of the fiber membrane to 2.70 MPa, and decreased the crystallinity. IR spectra showed that the addition of Pis-1 PG had no effect on the structure of PLA. In vitro antibacterial experiments revealed that Pis-1 PG/PLA fiber membrane could inhibit the growth of Shewanella putrefaciens within 6 hours, and exhibited a sustained antibacterial effect, with the antibacterial effect being significant (p<0.05). After 6 days of storage at 4 ℃, the total bacterial count of the fish treated with Pis-1 PG/PLA fiber membrane was lower (2.02~3.23 lg(cfu/mL)) than that of the blank group. As a result, Pis-1 PG/PLA fiber membrane inhibited effectively the increases of pH value and TVB-N content, and prolonged the cold storage period of sea bass by more than 3 days. In summary, Pis-1 PG was successfully loaded into PLA fiber membrane; Pis-1 PG/PLA fiber film exerted a preservation effect on sea bass thereby having the application potential in seafood preservation and food packaging.
    15  Changes in Contents of Quality Related Components of Tea Leaves under Different Preservation
    HU Die CHEN Wei MA Cheng-ying LYU En-li MIAO Ai-qing QIAO Xiao-yan
    2021, 37(4):110-115. DOI: 10.13982/j.mfst.1673-9078.2021.4.0916
    [Abstract](263) [HTML](158) [PDF 1.36 M](788)
    Abstract:
    In this work, the plucked tea leaves were preserved at different temperatures, including a range of refrigeration at 5~8 ℃, 10~13 ℃, and 15~18 ℃, as well as a room temperature storage at 21~23 ℃, for 36 hours. Changes of quality-related components of tea leaves were investigated during the process of preservation through detecting biochemical indexes. The content of soluble sugar showed no significant changes in neither refrigeration nor room temperature sample groups after 36 hour treatment. The content of free amino acids maintained at the same level before and after 36 hour refrigeration at 10~13 ℃. The catechin (C), epicatechin (EC), and epicatechin gallate (ECG) decreased significantly in all preservation treatments (p<0.05). The contents of epigallocatechin (EGC), gallocatechin (GC), and epigallocatechin gallate (EGCG) demonstrated no significant variation after 36 hour refrigeration at neither 5~8 ℃ nor 10~13 ℃. The result of principle component analysis (PCA) suggested all samples of four refrigeration treatments were separated from the room temperature ones on the basis of soluble sugar, free amino acids, C, EC, EGC, GC, EGC, and EGCG. The refrigerated samples also clustered closer to the fresh leaves than the ones of room temperature case. In conclusion, 10~13 ℃ treatment could be a better choice to store plucked tea leaves.
    16  Effect of Cold Atmospheric Plasma on Quality Maintenance of Penaeus vannamei during Cold Storage
    XU Hui-qian YAN Jin-hong TANG Ling-ling DENG Shang-gui MIAO Wen-hua
    2021, 37(4):116-123. DOI: 10.13982/j.mfst.1673-9078.2021.4.0831
    [Abstract](240) [HTML](285) [PDF 595.28 K](754)
    Abstract:
    In this study, Penaeus vannamei was used as the raw material for investigating the effect of cold atmospheric plasma sterilization technology on its quality changes during cold storage. Cold atmospheric plasma technology is a comprehensive sterilization technology that has emerged in recent years, and widely used in fields such as food, medicine and environment. The effects of cold atmospheric plasma processes (20 kV, 40 kV or 60 kV voltage for 3 min) on the quality changes of the Penaeus vannamei during cold storage were investigated including indices like the total plate count, total volatile base nitrogen (TVB-N), pH, 2-thiobarbituric acid (TBA), texture properties, color difference, and sensory attibutes). Experiments showed that the treatments with three different voltages could effectively slow down the deterioration of Penaeus vannamei, and the 60-kV treatment exhibited the strongest effect with the total plate count after 14 days of cold storage being 5.21 log(CFU/g) (which was lower than that 6.21 log(CFU/g) of the control group), and the TVB-N also higher than that of the control group over a certain time period. With the refrigeration time prolonging, the retention of the texture properties and color of the treated groups was significantly greater than that for the control group. The treatment slowed down significantly the increase of pH and proliferation of microorganisms, but promoted significantly the increase of TBA value. In general, the cold atmospheric plasma had a significant bactericidal effect on Penaeus vannamei during cold storage, and facilitate its quality maintenance.
    17  Analysis of the Stability of Whole Wheat Flakes Quality in Different Storage Conditions
    GAO Zi-hao YU Ke LIU Lei
    2021, 37(4):124-130. DOI: 10.13982/j.mfst.1673-9078.2021.4.0930
    [Abstract](250) [HTML](221) [PDF 589.41 K](558)
    Abstract:
    The purpose of this research was to evaluate the effect of storage conditions on nutrient loss and oxidation stability of the whole wheat flakes during storage, which could provide a theoretical basis for the prediction of the shelf life of whole wheat flakes. The whole wheat flakes was used as raw material, and the content of vitamin A, vitamin E, peroxide value, fatty acid value and acid value were measured under the conditions of 4 ℃, 25 ℃, 45 ℃ in dark and 25 ℃ with light for 30 days. The effect of temperature and light condition on nutrient and quality stability of whole wheat flakes were compared and analyzed. The results showed that lower temperature could significantly alleviate the nutrient loss and oxidative rancidity in whole wheat flakes. Among them, the nutrient loss of whole wheat flakes stored at 4 ℃ was the least and the oxidation stability was the best. Compared with the sample stored at 4 ℃, the contents of vitamin A and vitamin E of the sample stored at 45 ℃ decreased to 319.54 μg/100 g and 96.85 mg/100 g, respectively, and the decay rates were 2.5 and 3.3 times higher, respectively. The rate of lipid oxidation in wheat flakes was significantly reduced after low temperature treatment. Compared with storing at 45 ℃, the peroxide value, fatty acid value and acid value of samples stored at 4 ℃ after 30 days of storage decreased by 23.26%, 87.02% and 36.62%, respectively. In addition, light condition could induce decrease in the stability of whole wheat flakes significantly during storage. Compared with 25 ℃ in dark after 30 days storage, the contents of vitamin A and Vitamin E in whole wheat flakes that stored in light decreased to 400.16 μg/100 g and 180.19 mg/100 g, respectively and its peroxide value and acid value increased to 0.16 mmol/kg and 0.42 mg/g, respectively. In conclusion, whole wheat flakes stored at 4 ℃ and protected from light had the best quality stability.
    18  A Preliminary Study on the Regulating Mechanism of Vacuum Precooling on the Lignification of Mulberry Leaf Vegetables after Harvest
    YANG Teng-da CHEN Yu-long ZENG Fan-kun YE Ming-qiang WANG Ling LUO Zheng CHEN Fei-ping
    2021, 37(4):131-138. DOI: 10.13982/j.mfst.1673-9078.2021.4.0839
    [Abstract](265) [HTML](171) [PDF 1022.15 K](544)
    Abstract:
    To investigate the regulating effects and mechanism of vacuum precooling on the lignifying of mulberry leaf vegetables stored under shelf temperature, the changes of respiration intensity, vitamin C, lignin content, POD activity, LAC activity, relative conductivity and malondialdehyde (MDA) of mulberry leaf vegetables were studied in the present work. The results showed that the etiolation, vitamin C degradation and respiration rate of mulberry leaf vegetables were reduced by vacuum precooling treatment. Moreover, after storage for 6 days, the lignin content, POD activity and LAC activity of vacuum precooling treated samples were significantly lower than those of control, namely, 6.59 g/100 g vs 7.42 g/100 g, 36.33 U/g vs 46.93 U/g and 131.60 vs 146.40 U/g respectively. And the relative conductivity and MDA of vacuum precooling treated samples was 34.98% and 12.86% lower than those of control respectively. Based on these results, it can be reasonably deduced that the mechanism of vacuum precooling treatment on the lignification delay of mulberry leaf vegetables might be related to cell membrane damage reduction and polymerase activity inhibition. In conclusion, the results will be great important not only to deepen the scientific knowledge of quality deterioration of mulberry leaf vegetables after harvest, but also to provide technology theory for mulberry leaf vegetables industrialization.
    19  The Regulation of a Compound Color-protecting Agent on the Storage Quality of Fresh Spinach Noodles
    ZHAO Di WEN Qing-yu ZHANG Kang-yi ZHOU Yu-fei WANG Yan-fang ZHANG Li-zheng
    2021, 37(4):139-148. DOI: 10.13982/j.mfst.1673-9078.2021.4.0804
    [Abstract](274) [HTML](154) [PDF 969.80 K](590)
    Abstract:
    In this study, the effects of the amounts of sodium chloride, sodium D-isoascorbate and sodium copper chlorophyll on the color and quality of fresh spinach noodles were investigated using color difference and sensory score as the target parameters, along with the synergistic effect of the three additives on the color and quality of spinach fresh noodles. The study showed that under the conditions of 25 ℃ and 4 ℃, the optimized formula obtained through the orthogonal experiments was: 0.06% of sodium D-isoascorbate, 0.015% of sodium copper chlorophyll, and 1.50% of sodium chloride. The confirmatory experiments revealed that at the storage temperature of 25 ℃ and 4 ℃, the activities of polyphenoloxidase and peroxidase in the fresh spinach noodles were significantly inhibited compared with the control group; the total number of bacterial colonies in fresh spinach noodles reached 5.41±0.04 lg(CFU/g) during the 2-day storage at 25 ℃, but below 5.48 lg(CFU/g) during the 28-day storage at 4 ℃; For the same storage time, the total number of colonies were lower than that of the control group while the storage environment at 4 ℃ seemed more suitable for storing fresh noodles. In the meantime, the acidity of the optimal group was 1.47 mL/10 g within the 3-day storage at 25 ℃, and was 1.23 mL /10 g within the 21-day storage at 4 ℃, both of which were lower than that of the control group. In addition, the chlorophyll content and pH were higher than those of the control group, indicating that the compound color-protecting agent proposed in this study has a positive effect on the color and quality changes of fresh spinach noodles during storage. In this study, the influences of the compound color-protecting agents on the color and quality changes of fresh spinach noodles during the storage process were examined, which provides s theoretical support for the commercial development of agents for product color protection and shelf-life extension.
    20  Effects of Different Treatment Methods on Improving the Quality of Purple Sweet Potato Noodles
    FAN Hui-ping WU Dan AI Zhi-lu SI Yi-lei HOU Bing-jie ZHANG Bo-bo
    2021, 37(4):149-156. DOI: 10.13982/j.mfst.1673-9078.2021.4.0873
    [Abstract](255) [HTML](199) [PDF 10.68 M](942)
    Abstract:
    In this study, the changes of cooking loss rate, microstructure, rheological properties and resistant starch content of purple sweet potato flour were investigated to explore the effects of steaming, aging and freezing treatment on the quality of purple sweet potato noodle. The results showed that the cooking loss rate, resistant starch content, microstructure and rheological properties of purple sweet potato noodle prepared by extrusion changed under different treatment conditions including steaming, aging and freezing. The content of resistant starch gradually increased from 3.01% to 4.02% under 2 to 4 h aging treatment, but it remained unchanged with the further increasing of aging time. In the first 3 to 5 min, the content of resistant starch increased from 3.41% to 4.82% with the increase of steaming time, however, it decreased from 4.82% to 2.40% when the steaming time increased from 5 min to 11 min. Under different treatment conditions, the surface microstructure of purple sweet potato powder changed obviously, suitable steaming, aging or freezing treatment improved the microstructure of purple sweet potato noodle, but overtreatment also led to deterioration of the microstructure. The storage modulus and loss modulus of untreated purple sweet potato noodle were obviously higher than those of treated purple sweet potato noodle, and the storage modulus was obviously higher than the loss modulus, and the elastic modulus was dominant. Therefore, suitable steaming, aging and freezing treatment can effectively improve the whole quality of purple sweet potato noodle.
    21  Comparison of the Quality of Different Varieties of Yingde Black Tea
    SANG Jia-qi WEN Jing LIU Hao-cheng XU Yu-juan XIAO Geng-sheng WU Ji-jun FU Man-qin BU Zhi-bin LAN Li-dan HUANG Hui-xin
    2021, 37(4):157-162. DOI: 10.13982/j.mfst.1673-9078.2021.4.0748
    [Abstract](419) [HTML](154) [PDF 683.23 K](701)
    Abstract:
    In this study, four common varieties of Yingde black tea (A, B, C, D) were used as the research objects. The quality of the four types of tea was compared through measuring the contents of tea polyphenols, amino acids, water-soluble sugars, theaflavin, and antioxidant activity as well as sensory quality of tea. The results showed the content of tea polyphenols, water extract content, content of amino acids and theaflavin content in the four kinds of black tea were in the range of 14.53~21.98%, 29.57~38.56%, 2.85~3.04% and 1.57~3.75%, respectively. Among which, the contents of tea polyphenols and water extract in No. A were significantly (p<0.05) higher than those of No. C and No. B; the content of amino acids in No. C was significantly (p<0.05) higher than those of No. A and No. B. The content of theaflavins was the highest (3.75%) and lowest (1.57%), respectively in No. A and No. B tea. The content of catechin in four kinds of black tea ranged from 9.95% to 16.98%, with gallocatechin gallate possessing the characteristic tea soup flavor accounting for 5.98%~9.21% of the total content. Among the four black tea extracts, No. D had the highest antioxidant activity, and No. C had the lowest antioxidant activity, with a correlation between the antioxidant activity and polyphenols. The results of the artificial sensory evaluation showed that the taste and attribute characteristics of the four tea samples differed, with the No. C superior to the other three kinds of tea in attributes of taste, aroma and leaf bottom, followed by No. A. Overall, although the four kinds of Yingde black tea exhibited differences in the content and sensory characteristics of biochemical components, they all had the characteristic quality of Yingde black tea soup i.e. bright red color and strong sweet fragrance. No. A and No.C tea possess higher tea quality.
    22  Quality Changes of Frozen Prepared Fotiaoqiang Product Reheated by Different Methods
    HU Qin HUANG Xu-hui QI Li-bo FU Bao-shang DONG Xiu-ping QIN Lei
    2021, 37(4):163-171. DOI: 10.13982/j.mfst.1673-9078.2021.4.0803
    [Abstract](374) [HTML](197) [PDF 785.15 K](775)
    Abstract:
    Three different reheating methods, including water bath heating, steam heating, and microwave heating were used to reheat the frozen prepared Fotiaoqiang product to investigate their influences on the quality of Fotiaoqiang. Using fresh Fotiaoqiang finished product as the control, the color, MDA content, free amino acid, 5’-nucleotide content, texture, taste perceived by electronic tongue and sensory score of the reheated product were measured to determine the best reheating method. The results showed that the sensory score of the product after steam reheating was the highest. Different reheating methods have little effect on the texture characteristics of the main material abalone. In terms of color, the difference in the a*value of the products subjected to three different reheating methods was insignificant. The L*value and b*value of the product subjected to microwave reheating differed significantly from the control group. In terms of fat oxidation, the MDA value of the microwave reheated product was the highest (2.86 mg/kg; increase by 36.84% compared with the control group). Electronic tongue analysis found that the saltiness of the control group and the microwave reheated product was 3.45 and 4.34, respectively which achieved an (increase by25.79%). Compared with the control group, the contents of free amino acids and 5’-nucleotides in the soup increased for the microwave reheated product but decreased for the products subjected to water bath and steam reheating. Taken together, steam reheating was an ideal reheating method for frozen prepared Fotiaoqiang product.
    23  Xanthan Gum and Konjac Gum Inhibits Liquefaction of Alkali-induced Duck Egg White Gelsat High Temperature
    FAN Hong AI Min-min CAO Yuan-yuan GUO Shan-guang LONG Jiao-li LI Shu-chang JIANG Ai-min
    2021, 37(4):172-179. DOI: 10.13982/j.mfst.1673-9078.2021.4.0879
    [Abstract](304) [HTML](212) [PDF 2.14 M](705)
    Abstract:
    Xanthan gum and konjac gum were added into duck egg whites to prepare alkali-induced gels to explore the inhibitory effect of hydrocolloids on liquefaction of egg white gels at high temperature. Results showed that the viscosity, storage modulus and loss modulus of prepared egg white gel with adding hydrocolloids increased obviously (p<0.05), and the browning intensity increased by 10.40% and 17.20%. When the concentration of xanthan gum and konjac gum was increased from 0.50% to 1.50%, the hardness improved by 49.60% and 119.56%, the puncture strength increased by 20.59% and 78.42%, the water-holding capacity enhanced by 1.02% and 9.47%, respectively, but the hardness, puncture strength and water holding capacity of the gel added with xanthan gum were significantly greater than those of adding konjac gum. When the concentration of the two glues is 1.00%, the sensory score of the egg white gel is the highest. The addition of these two hydrocolloids changed the secondary structure of the protein and the intermolecular forces of gel: The addition of xanthan gum weakened the ionic interaction (p<0.05), and the proportion of random curls reduced by 41.23%, α-helixes increased by 81.29%; While the addition of konjac gum reduced the hydrophobic interaction (p<0.05), and the β-sheets content decreased by 34.97%, the α-helices improved by 68.97% and 70.37%, respectively; And the proportion of hydrogen bonds and disulfide bonds improved with the increase of these two hydrocolloids. In summary, the addition of xanthan gum and konjac gum could inhibit the liquefaction of gel after high-temperature treatment, and the textural characteristics and water-holding capacity of the gel with adding xanthan gum are better than konjac gum, but konjac gum showed a more remarkable influence on the browning intensity than xanthan gum.
    24  Changes in Color and Extent of Yolk Coagulation of Characteristic Yellow Preserved Eggs of Sansui in Guizhou during Lead-free Curing
    CHEN Yan-hong WANG Xiu-jun XU Wen LIU Lin-xin YANG Li-ping YU Pei NIE Qian-li YANG Wan-yun YAO Bi-qiong FENG Ting-cui
    2021, 37(4):180-188. DOI: 10.13982/j.mfst.1673-9078.2021.4.0822
    [Abstract](199) [HTML](359) [PDF 372.69 K](374)
    Abstract:
    In order to study the changes in the color and coagulation extent of preserved eggs during lead-free curing process and on the basis of the lead-free curing technology for yellow preserved eggs, the copper sulfate-zinc sulfate compound was used in the place of lead oxide, and the effects of the concentration of Cu-Zn compound solution, the Cu-Zn ratio, the curing temperature, the NaOH concentration and the curing time on the color and coagulation degree of preserved egg yolks were investigated. The results showed that the curing temperature, NaOH concentration and curing time were the main influencing factors, and on the basis of studying the main factors, the optimum curing conditions for yielding characteristic yellow preserved eggs with the most desirable color, yolk coagulation extent and sensory quality were: concentration of copper-zinc compound solution, 0.40%; copper-zinc ratio, 1:2; curing temperature, 15 ℃; NaOH concentration, 4%; curing time, 18 days. Under such curing conditions, the coagulation extent of the preserved egg yolk was 0.85, the whiteness (W) of egg white was 30.15, and the whiteness (W) of the yolk was 46.72. The preserved egg was translucent yellow, the gel was intact with moderate softness and hardness and glossy appeal. The quality of the preserved egg was excellent, with a sensory score of 81.35 and comprehensive score of 0.92. In summary, this research provides a theoretical basis for the production of lead-free preserved egg.
    25  Optimization of Color Retaining Agent and Non-enzymatic Browning Analysis of Liguang Apricot Drying Process
    CAO Jia-le CHI Yan SUN Jing-yao ZHANG Bing WANG Xin YANG Hai-yan
    2021, 37(4):189-198. DOI: 10.13982/j.mfst.1673-9078.2021.4.0884
    [Abstract](204) [HTML](152) [PDF 1.92 M](776)
    Abstract:
    To improve the color and lustre of Liguang apricot during the traditional drying process and explore the change law of main substances related to non-enzymatic browning, the Liguang apricot produced in Xinjiang Province was taken as the material for study. The effect of citric acid, phytic acid and L-cysteine on the color and lustre of dried Liguang apricot was analyzed and studied. On the basis of the single factor experiment, the optimum concentration of the soaking liquid was determined through the Box-Benhnken experiment, with L* value as the indicator. The content of ascorbic acid, total phenols, free amino acid and reducing sugar and the variation of L* value during the drying process of the Liguang apricot which had been processed with compound color fixative were studied. Also, the non-enzymatic browning parameters were analyzed through correlation analysis. The results showed that the impact degree of the factors was as follows: phytic acid > L-cysteine > citric acid, and the best compound effect came from the following concentration combination: 0.60% of citric acid, 0.06% of phytic acid and 0.20% of L-cysteine. The measured value of L* was 42.18±0.22 with a good color protection effect; after drying, the contents of ascorbic acid, reducing sugars, total phenols, and free amino acids of dried apricot slices decreased by 85.68%, 36.53%, 64.00%, and 23.03% respectively, compared with fresh fruits. In conclusion, the compound sulfur-free color fixative can effectively inhibit the Maillard reaction and the oxidation of ascorbic acid of Liguang apricot during its drying process and improve the color and lustre of the dried Liguang apricot.
    26  Preparation of Acetylated Citric Acid Esterified and Cross-linked Mechanically Activated Starch and the Analysis of Their Properties
    WANG Ji-wei NING Yu-qi HU Hua-yu ZHANG Yan-juan HUANG Zu-qiang LIANG Jing
    2021, 37(4):199-206. DOI: 10.13982/j.mfst.1673-9078.2021.4.0837
    [Abstract](197) [HTML](430) [PDF 1.29 M](697)
    Abstract:
    Acetylated citric acid esterified and cross-linked starch was prepared by the solvent method, with mechanically activated cassava starch as material, acetic anhydride as esterification agent, and citric acid as cross-linking agent. The effects of mechanical activation time, amount of mixed acids, reaction temperature, reaction time, and mass ratio of citric acid to acetic anhydride on the sedimentation volume of acetylated citric acid esterified and cross-linked starch were investigated. The structure and properties of the as-prepared esterified and cross-linked starch were characterized and determined. The results showed that the optimum technological conditions were as follows: mechanical activation time of 40 min, addition amount of mixed acid of 10% (accounting for dry weight of starch), reaction temperature of 45 ℃, reaction time of 1 h, and the mass ratio of citric acid to acetic anhydride of 1:35, the sedimentation of product was 1.52 ml. FT-IR showed that the starch was successfully esterified and cross-linked. X-ray diffraction (XRD) showed that the esterification and cross-linking reactions occurred in the amorphous region of starch. The results showed that the order of water extraction rate and paste transparency is as follows: mechanical activated starch > raw starch > esterified and cross-linked mechanically activated starch > esterified and cross-linked starch, which indicates a better freeze-thaw stability and a lower paste transparency of esterified and cross-linked starch compared to those of raw starch. In addition, the order of acid-resistance properties is as follows: esterified and cross-linked mechanically activated starch > mechanically activated starch > esterified and cross-linked starch > original starch, indicating that mechanical activation can improve the acid-resistance property of starch. Furthermore, the viscosity thermal stability of esterified and cross-linked starch is better than that of raw starch. In general, the mechanical activated esterification cross-linked starch displays a better freeze-thaw stability, acid-resistance property and viscosity thermal stability.
    27  Comparison of Microstructure and Quality of Instant Noodle Prepared with Different Drying Methods
    HUANG Ting-ting FENG Xin FENG Zuo-shan BAI Yu-jia FU Wen-qian
    2021, 37(4):207-216. DOI: 10.13982/j.mfst.1673-9078.2021.4.0944
    [Abstract](308) [HTML](249) [PDF 6.74 M](777)
    Abstract:
    The effects of five different drying methods [Hot air drying (HAD), freeze-drying (FD), microwave dry (MD), Microwave vacuum drying (MVD), hot air followed by microwave drying (HAD+MD)] on the physical properties and microstructure of instant noodle were investigated. The results showed that the order of different drying rates was as follow: MVD > MD > HAD+MD > HAD > FD. The order of rehydration rate was as follow: FD > MVD > MD > HAD+MD > HAD. There was no significant difference in total color difference between FD group and MVD group (p>0.05). The total color difference of HAD was 19.56, and the color of the surface was poor. There was no significant difference in total color difference between HAD+MD group and MD group (p>0.05). After rehydrated for 4 min, the texture analysis showed that the MD and MVD groups had the best ductility (81.58 g/sec and 72.58 g/sec, respectively), while the shear force of the FD group showed poor performance. The hardness and mastication of the HAD group were relatively large (1652.88 g and 5294.77 g, respectively). The results of scanning electron microscope showed that uniform and dense pore structure was observed in both FD group and MVD group. The non-uniform pore structure was observed in MD group and HAD+MD group, while the pore structure was hardly observed in HAD group. The results of sensory evaluation showed that FD group was the best, MVD and MD slice was the second, HAD+MD and HAD slice was the worst. In conclusion, MVD drying method could be used as a suitable dehydration method to develop the noodle in Xinjiang soup noodle products.
    28  Comparison of the Physicochemical Properties of Clear Beetroot Juice-Enriched Noodle with Different Amounts of Alkali
    SHIAU Sy-yu LI Ying PAN Wei-chen
    2021, 37(4):217-223. DOI: 10.13982/j.mfst.1673-9078.2021.4.0829
    [Abstract](205) [HTML](185) [PDF 681.15 K](604)
    Abstract:
    Beetroot juice, rich in bioactive substances, is a good source of food colorings and functional ingredients. In this study, clear beetroot juice (CBJ) was added to the formulated noodle to improve the nutritional value, antioxidant properties and color of the noodle. The 10% CBJ-noodles was used as the research object to study the effects of added alkali level (0~0.40%) on the texture, color and antioxidant properties of the resultant noodles. Texture and color of the noodles were measured by a texture analyzer and a colorimeter, respectively. Total contents of betalains and polyphenols, and the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity were determined by spectrophotometry. The research results showed that compared with the control noodle (without alkali), increasing the alkali level (0.10~0.40%) significantly increased the breaking force (11.07~33.35%) and the content of free polyphenolics (2.26~25.28%) of the dried CBJ-noodles, whilst decreasing significantly total betalains (3.77~43.20%) and DPPH radical scavenging capacity (1.08~18.20%). In addition, increasing the level of added alkali increased significantly the cutting force (10.88~29.23%) and tensile strength (47.78~137.21%) of cooked noodles. In terms of color, the redness (a* value) of dried raw and cooked CBJ-noodle with 0~0.40% alkali ranged from 16.59 to 1.98 and from 7.39 to 1.76, respectively. These results indicate that the betalains in CBJ were unstable at a high pH and temperature. Therefore, this research proposes the use of a low alkali level (0.10%) for the preparation of alkaline noodles, which makes the noodles not only having a pink appearance but also possessing higher contents of retained betalains and polyphenols and higher antioxidant activity.
    29  Optimization of Fermentation Process for Hongyang Kiwifruit Jiaosu and Antioxidant Activity in Vitro
    CHEN Lin SU San WU Ying-mei XU Feng-lian CHENG Yang-qing
    2021, 37(4):224-233. DOI: 10.13982/j.mfst.1673-9078.2021.4.0840
    [Abstract](290) [HTML](144) [PDF 988.76 K](811)
    Abstract:
    The fermentation process of Hongyang kiwi jiaosu produced in Chongqing was optimized by biorthogonal experiment with hydroxyl radical scavenging rate (50%) and sensory evaluation (50%) as comprehensive indexes. Firstly, the addition of yeast (Saccharomyces cerevisiae), lactobacillus (Lactobacillus bulgaricus & Streptococcus thermophilus), pectinase and cellulase were chosen as the independent variables and optimized through the first orthogonal experiments on the basis of single-factor experiments. The addition amounts of yeast, Lactobacillus, pectinase and cellulase were determined to be 2.0%, 2.0%, 0.5% and 2.0%, respectively. Then on this basis, the initial amount of sugar, fermentation temperature, fermentation time and solid-liquid ratio were investigated through the second orthogonal test as another part of the independent variables. The optimal technological parameters were as follows: 20% of initial amount of sugar, 1:1.3 of solid-liquid ratio, and fermentation at 36 ℃ for 35 d, under these conditions the hydroxyl free radical clearance rate, sensory evaluation and comprehensive indexes were 94.33%, 76.33 and 85.33, separately. The results showed that the fermentation process of Hongyang kiwifruit jiaosu could be optimized by the orthogonal experiment. In addition, it was found that the antioxidant activity of the fermentation product was significantly higher than that of the natural fermentation group (p<0.05), and the hydroxyl free radical clearance rate, DPPH·, ABTS·+ were increased by 8.69%, 11.89% and 36.57%, separately. This study provides a new way to promote the deep processing of Hongyang kiwifruit with local characteristics.
    30  Regulation of Aroma and Color Characters in ‘Cabernet Sauvignon’ Wines through ‘Coupage’ Technique
    LING Meng-qi ZOU Wen-wen WU Guang-feng YANG Wei-ming DUAN Chang-qing SHI Ying
    2021, 37(4):234-241. DOI: 10.13982/j.mfst.1673-9078.2021.4.0877
    [Abstract](260) [HTML](187) [PDF 1.55 M](844)
    Abstract:
    To avoid deficiencies in monovarietal wines and improve flavor quality by blending different wines with different proportion through the technique of wine blending after fermentation (‘coupage’), Cabernet Sauvignon wine was regarded as base wine and blended with Cabernet Franc, Marselan, and Petit Verdot wines with proportion of 10%, 20%, 30%, respectively. Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography triple quadrupole mass spectrometry (HPLC-QqQ-MS/MS) were used to detect flavor compounds, namely, volatile aroma compounds and phenolic compounds. Quantitative descriptive analysis (QDA) and CIELAB model were conducted to evaluate the difference of wine sensory quality. The results showed it was not an efficient way to increase fruity aroma by merely blending wines with high level of esters. Only Cabernet Franc wine with proportion above 20% increased the concentration of acetates in blending wine samples after 6-month bottle aging (p<0.05). Marselan wine with proportion over 10% could promote flower attribute and reduce herbal notes by increasing concentration isoprenoids, and lower the yellow hue at the same time. Petit Verdot wine with proportion of 30% had great contribution to the improvement of phenolic composition, especially by increasing the concentration of flavanols (1.43 times), flavonols (1.27 times) and phenolic acids (4.68 times), which was beneficial for color stabilization. Therefore, it is practicable to regulate aroma and color characteristics in ‘Cabernet Sauvignon’ wines through wine blending after fermentation, which provides theoretical basis for scientific design of ‘coupage’.
    31  Drying Characteristics and Flavor Changes of Catfish Fillets under Different Drying Methods
    ZHOU Ming-zhu XIONG Guang-quan QIAO Yu LIAO Li WANG Lan WU Wen-jin LI Xin SHI Liu DING An-zi LI Cai
    2021, 37(4):242-251. DOI: 10.13982/j.mfst.1673-9078.2021.4.0905
    [Abstract](293) [HTML](203) [PDF 1.29 M](762)
    Abstract:
    In this paper, the effects of different drying methods on the drying characteristics of catfish were studied. Gas chromatography ion mobility spectrometry (GC-IMS), electronic nose, electronic tongue and amino acid analyzer were used to analyze the flavor of different drying methods. The results showed that the shrinkage rate of vacuum freeze-drying was 5.44%, which was significantly lower than the other three drying methods (p<0.05), and its rehydration rate was the fastest and the time was the shortest; the hardness of hot-air drying was the highest, reaching 1929.70 g. Compared with the other three drying methods, there was a significant difference (p<0.05); the L* value was the minimum, a* value and b* value were the largest in the vacuum drying, which made the catfish slice appear caramel color, while the vacuum freeze-drying had the highest L* value, the lowest a* value and b* value, which made it white; the hot-air drying and microwave drying had similar odor, and the hot-air drying and vacuum drying had similar odor. The sweet amino acid content of hot air drying and vacuum freeze-drying were 51.97 mg/g and 67.66 mg/g, respectively, higher than those of bitter amino acids; the contents of bitter amino acids in microwave drying and vacuum drying were 3.56 mg/g and 6.11 mg/g, respectively, higher than those of sweet ones. Therefore, the vacuum freeze-drying had the least effect on the drying characteristics of channel fish fillets, and the flavor was different from the other three drying methods.
    32  Development of a Time-Resolved Fluorescence Immunochromatographic Assay for the Quantitative Determination of AFB1 and ZEN in Grain
    WANG Xu LU Di-sha ZENG Dao-ping HUANG Jian-xin WU Ying XU Xiao-xuan TAN Shu YANG Jin-yi
    2021, 37(4):252-261. DOI: 10.13982/j.mfst.1673-9078.2021.4.0881
    [Abstract](349) [HTML](243) [PDF 68.78 M](854)
    Abstract:
    A time-resolved fluorescence immunochromatographic assay for the quantitative determination of AFB1 and ZEN in grain was developed. Anti-AFB1 and anti-ZEN were labelled with time-resolved fluorescent microsphere as tracers. The assay conditions, including pH value of labelling, concentrations of antibody for labeling, concentration of the coating antigen (T line) and the goat-anti-mouse IgG (C line), as well as the sample pretreatment were optimized. Under the optimal condition, the assay showed the AFB1 limit of detection and detection range were 0.80 ng/mL and 0.81~5.67 ng/mL, respectively; the AFB1 IC50 was 2.15 ng/mL; the assay showed the ZEN limit of detection and detection range were 4.58 ng/mL and 4.76~85.60 ng/mL, respectively, the ZEN IC50 was 20.19 ng/mL. The cross-reaction rates with T-2、DON、FB1、OTA were less than 10%, indicating a good specificity of the proposed assay. Recovery test was done by using maize, soybean, wheat bran and wheat sample. The AFB1 recoveries ranged from 97.10% to 108.7% with RSDs below 15% and the ZEN recoveries ranged from 92.8% to 109.1% with RSDs below 15%. The results of the FAPAS had a good agreement with those of UPLC-MS/MS. The results had a good agreement with colloidal gold immunochromatography and ELISA. In conclusion, theproposed assay was convenient、rapid、accurate and sensitive. The detection process is about 25 min. It is suitable for rapid screening of AFB1 and ZEN in grain samples.
    33  The Active Components of Testosterone Secreted by Antler Testis Stromal Cells Rapidly Screened by Electrospray Tandem Mass Spectrometry and Chemometrics
    WANG Ya-ping LI Jing-feng ZHANG Kai-yue ZHANG Nan-xi GAO Ying ZHANG Hui SUN Jia-ming
    2021, 37(4):262-268. DOI: 10.13982/j.mfst.1673-9078.2021.4.0972
    [Abstract](201) [HTML](202) [PDF 747.54 K](558)
    Abstract:
    Electrospray tandem mass spectrometry (ESI-MS/MS) and chemometrics were used to screen the active components of testosterone secreted by testis stromal cells of Antler antler. Using electrospray tandem mass spectrometry, the chemical composition of 6 batch of pilose antler and 6 batch of velvet antler prepared by traditional process was detected. The testosterone secretion of rat leydig cells was also determinated. The principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the mass spectrometry data. Rapid differentiation of fresh velvet antler and traditional processed products was achieved. Partial least square method (PLS) and gray correlation degree analysis (GRA) were used to select the active components with potential testosterone secretion by testicular stromal cells. The structure and composition of the possible active components were determined by secondary mass spectrometry data analysis. The results showed that the components of fresh antler and traditional processed products were significantly different, and both of them had good activity of testosterone secretion by testicular stromal cells. The most active components were Tyr-Ser-Phe (m/z 416), PHE-Phe-Leu/Ile (m/z 426), respectively. This method could be used to analyze the active components of testicular stromal cells in antler and provide reference for the development of antler as health food.
    34  Rapid Detection of Glucosinolates in Cauliflower Based on Visible/Near-infrared Spectroscopy
    HUANG Liang XUE Jian-xin MU Bing-yu
    2021, 37(4):269-274. DOI: 10.13982/j.mfst.1673-9078.2021.4.0874
    [Abstract](316) [HTML](182) [PDF 521.57 K](613)
    Abstract:
    In order to quickly detect the content of glucosinolates in different varieties of cauliflower (“Songhua” and “Snow-white”), in this experiment, fresh "Songhua" and "Snow-white" cauliflower samples were collected for visible/near-infrared spectrum collection, extraction and analysis. First, five methods, Baseline correction, Standard Nomal Variate transform (SNV), Median Filter (MF), Gaussion Filter (GF) and S-G smoothing (savitzky-golay), were used for the preprocessing analysis of the original spectra. Secondly, methods of extracting feature bands were respectively adopted, namely the Successive projection Algorithm (SPA) and the Regression Coefficient (RC) were used to extract the feature bands. Principal Component Analysis (PCA) was used to extract the Principal components. A Partial Least Squares Regression (PLSR) model was established based on the optimal preprocessing method. The results showed that the MF-PCA-PLS model established by the spectral data of "Songhua" cauliflower was the best, and the calibration set model parameter Rc=0.89, RMSEC=1.23, predictive set model parameters Rp=0.89, RMSEP=0.63.The MF-RC-PLS model based on the spectral data of "Snow-white" cauliflower was optimal, with calibration set model parameters Rc=0.87, RMSEC=1.31, predictive set model parameters Rp=0.73, RMSEP=0.46. It can be seen that the content of glucoside in cauliflower can be detected quickly, nondestructively and accurately by PLSR algorithm combined with visible/near-infrared spectroscopy.
    35  Development of Detection Method of Salmonella in Food by Saltatory Rolling Circle Amplification Combined with FTA Card
    ZHUANG Meng-qing ZHANG Xian-zhou LU Xin GUO Wei MA Xiao-yan ZHANG Wei
    2021, 37(4):275-283. DOI: 10.13982/j.mfst.1673-9078.2021.4.0903
    [Abstract](318) [HTML](241) [PDF 2.25 M](775)
    Abstract:
    In order to achieve the simple and rapid on-site detection of Salmonella in food, saltatory rolling circle amplification (SRCA) method combined with FTA card (FTA-SRCA) was used to establish a new detection method for Salmonella. The template DNA was extracted by FTA card quickly. According to the specific invA gene of Salmonella, primers were designed and screened. Based on the above, the FTA-SRCA reaction system was established. The reaction was amplificated in the well plate which can achieve intensification. After the reaction, fluorescent dyes were added to observe the results. Its specificity, sensitivity and detection limit of artificial contaminated samples were determined. Furthermore, 60 actual samples were tested to evaluate relative sensitivity, specificity and accuracy. The results indicated that the 17 strains of Salmonella tested were all positive, and the 29 strains of non-Salmonella were negative. Therefore, the method had good specificity. The sensitivity of the FTA-SRCA was 6.81×100 CFU/mL, which was 100 times that of the PCR method and 10 times that of the SRCA method For the detection of artificially contaminated milk samples, the detection limit of the FTA-SRCA was 3.22×100 CFU/mL, which was 1000 times that of the PCR method and 10 times that of the SRCA method. The sensitivity, specificity and accuracy of FTA-SRCA were 100.00%, 94.64% and 95.00%, respectively. The FTA-SRCA method established in this study had the advantages of simple and rapid operation, low cost, strong specificity, high sensitivity, and low detection limit, which can be used for the intensive and rapid on-site detection of Salmonella in food.
    36  Research and Development of Compound Calcium Preparation and Construction of Evaluation System
    LI Hai-zhi LIU Yi-feng PAN Cong YU You-qiang CUI Li-zhu PANG Bin HE Xie-xun XIA Kai
    2021, 37(4):284-291. DOI: 10.13982/j.mfst.1673-9078.2021.4.0882
    [Abstract](244) [HTML](179) [PDF 1.71 M](645)
    Abstract:
    In order to develop a calcium supplement product with good solubility and easy acceptability, through the evaluation of other raw materials, a compound calcium preparation product was obtained. Finally, the high-speed granulation process was used to improve the liquidity of compound calcium preparation, and the electronic nose and electronic tongue were used to evaluate the compound calcium preparations. The results showed that after fluidized bed granulation, the fluidity of the compound calcium preparation was significantly improved, and the fluidity index increased from 67.00±0.00 to 80.00±0.00. The particle size of the powder after granulation is mainly distributed around 300.00 μm. Its wettability is good in 10.25±0.05 s. Compared with the ungranulated compound calcium preparation, the solubility of the granulated compound calcium preparation is enhanced; and the compound calcium preparation has suitable sour taste and umami taste, lower bitterness value and better taste; its smell can also be distinguished from other calcium supplement brands. Through the physical properties and sensory evaluation, it is concluded that the compound calcium preparation developed is a calcium supplement product that has good fluidity, good taste and smell, and easy to be accepted. At the same time, the physical and sensory properties of compound calcium preparation and two kinds of calcium supplement products with good sales were compared, and a set of complete evaluation system was established.
    37  Optimization of Key Sensory Properties of Sterilized Yogurt Based on Regional Preference
    CONG Yi-jie MA Rui
    2021, 37(4):292-301. DOI: 10.13982/j.mfst.1673-9078.2021.4.0869
    [Abstract](299) [HTML](282) [PDF 2.10 M](881)
    Abstract:
    The sensory preference and key sensory attributes of sterilized yogurt were tested for consumers in the three representative cities in the three regions of sterilized yogurt consumption in northern China. The sensory evaluation results were analyzed by multivariate statistical analysis, partial correlation analysis and penalty analysis. It was found that there have same cognitive trend towards sterilized yogurt in Qingdao, Shijiazhuang, Xi'an consumers. It showed that the consumers in Qingdao prefer products with weak acidity, frankincense and viscosity, the consumers in Shijiazhuang prefer products with weak viscosity, weak frankincense and strong acid taste, and the consumers in Xi'an prefer products with strong frankincense flavor and weak viscosity. The ordering of the overall preference of 4 kinds of normal temperature yogurt in the three regions is slightly different. On the whole, the preference of samples A and C is significantly higher than that of samples B and D (p<0.05). The results showed that the taste of normal temperature yogurt in different regions was different, the improvement degree and direction of the sensory quality of the sample were determined through penalty analysis to meet the needs of different regional consumer tastes.
    38  Research Progress on Inactivation of Bacterial Spores by Cold Plasma Technology
    CHENG Jun-hu ZHANG Yan HAN Zhong
    2021, 37(4):302-310. DOI: 10.13982/j.mfst.1673-9078.2021.4.0342
    [Abstract](474) [HTML](206) [PDF 1.41 M](1105)
    Abstract:
    Spores represent the dormant form of bacteria that are often found in foods, and usually resistant to stress and difficult to inactivate, which poses challenges to food safety. Cold plasma, as a new non-thermal physical sterilization technology, has been gradually developed in recent years. This technology inactivates microorganisms through stimulating gas to generate a variety of active species with high sterilization efficiency, short treatment time, environmental friendliness and minimal pollution. This review summarized the principle of cold plasma production, the influence of plasma on the internal and external structures of spores and the factors affecting the inactivation of spores, which provides solutions and technical support for the treatment of bacterial spores in food, and is conducive to promoting the industrial application of cold plasma in the field of food microbial sterilization.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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