Abstract:
In this paper, the diversity distribution of kiwifruit soft rot diseases in main kiwifruit producing areas in Hunan Province was systematically analyzed. It provided basic data and theoretical basis for further study on the diversity of pathogenic fungi of kiwifruit soft rot in Hunan. Illumina HiSeq high-throughput sequencing technology was used to collect the second generation sequencing data of microbial ITS amplifiers from 15 groups of fruit samples of 5 soft-rotting kiwifruit (A, B, C, D, E), and bioinformatics analysis was performed. There were abundant fungi in five soft rotting kiwifruit fruits, and the fungi richness index and diversity index were higher, especially for A, B and C groups, relative to D and E groups, there were significant differences (p<0.01).Two dominant Phylum microorganisms were obtained from different kiwifruit fruits. Ascomycota was the most dominant phylum. It mainly distributed in D and E groups. The relative abundance of Ascomycota
was 19.20%, which was much larger than that of Basidiomycota, 6.80%, which mainly distributed in A, B and C groups. It showed obvious advantages. The relative abundance of Diaporthe was the highest, It reached 14.00%, which was significantly different from other pathogens (p<0.01), The content of the bacteria was relatively high in groups of D and E, which reached more than 30% in these groups, and almost none in the other three groups. Next, Bullera was 4.90%, Groups A, B and C were slightly higher, reaching about 10% in these groups and about 5% in groups of D and E. Neofusicoccum was 3.20%, The content of the bacteria in Group E was relatively high, reaching more than 20% in this group, and almost none in the other four groups. Russula was 0.60%, and Tomentella, Phialocephala, oidiodendron and Cenococcum were few, among them, the genus of Homa, Penicillium and Simplicillium was the potential fungi for soft rotting kiwifruit fruit. The experiment provided a theoretical basis for the control of kiwifruit soft rot. In the future, the control of kiwifruit fruit soft rot disease can be targeted to kill the microorganisms of Diaporthe and Neofusicoccum.
