Volume 29,Issue 11,2013 Table of Contents

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  • 1  Effect of Enzymatic Modification on Binding Capacity between Pigment and Glycinin
    YANG Xiao-quan YAN Jiang-yin HOU Jun-jie
    2013, 29(11):2566-2570.
    [Abstract](1157) [HTML](0) [PDF 818.65 K](636)
    Abstract:
    To enhance photostability of Monascus pigment, glycinin was modified via glutamyl endopeptidase (GE), which had strict substrate specificity. The surface hydrophobicity of the 11S globulin after hydrolysis was analyzed, and protein/pigment complex was produced after enzymatic modification of the 11S. The binding properties (binding constant and binding capacity) and the light stability of protein/pigment complex were investigated. The results showed that GE had a significant effect on 11S surface character and Monascus pigment photostability. Surface hydrophobicity of 11S increases with the degree of hydrolysis (DH) increased after GE modifying as the inner hydrophobic groups exposed. At pH 7.0, the binding constants of Monascus pigment and 3% (m/V) 11 S reached the maximum of 215.00 U/g pro when the DH was 1.50%. After 24 h light exposure, the protein/pigment complex had the color value retention rate of 90% which highly improved the light stability of Monascus pigment
    2  Physicochemical Properties and Digestibility in Vitro of Non-crystalline Granular Corn Starch
    ZHANG Li-ming SHAN Jing HU Xiao-ding DAI Yu-jie LU Fu-ping
    2013, 29(11):2571-2574.
    [Abstract](1371) [HTML](0) [PDF 510.91 K](556)
    Abstract:
    Non-crystalline granular starch (NCGS) is a special structural starch, possessing granular integrity but not crystalline. In order to modify the properties of native starch granules, NCGS was prepared by ethanol solutions. Effects of non-crystalline treatment on physicochemical properties of starch, such as granular appearance, crystallinity, solubility, swelling power and in vitro digestibility were studied. The effects of this modification on rapidly digestible starch (RDS), slowly digestible starch (SDS), and resistant starch (RS) were also studied. The results showed that the granularity was kept integrated while the granules started to depress from the hilum region leading to substantial wrinkling on the surface. The treated NCGS showed V-type X-ray diffraction patterns, and its crystallinity was almost disappeared. The granules structure changed to non-crystalline. The solubility and swelling power of NCGS were increased compared with the native starch. The RDS levels of NCGS decreased from 92.83% to 81.64%, while the SDS+RS levels increased by 7.17% to 18.36%. Therefore, preparation of NCGS from aqueous ethanol treatments may be useful in the development of new products with low calorie and slow glycemic response.
    3  Expression of Trehalose Synthase in Pichia pastoris
    WANG Rui-ming LI Zhong-kui WANG Teng-fei LIU Hong-juan LI Pi-wu
    2013, 29(11):2575-2579.
    [Abstract](1350) [HTML](0) [PDF 489.79 K](572)
    Abstract:
    The expression vector was constructed from trehalose synthase (TreS) gene(AE015451.1)of Pseudomonas putida to express in Pichia pastoris. TreS gene was amplified by PCR and double digested by EcoRⅠ/XbaⅠ, then linked to the pPICZaA which digested with same enzymes. The gene was identified, sequenced and transformed into pichia pastoris GS115 by electroporation. Positive colonies harboring target genes were screened out by the YPD medium with Zeocin. The genome of positive colonies were extracted and a same size band with target gene was abtained by using PCR which illustrated target gene had successfully transferred to Pichia pastoris. SDS-PAGE and HPLC results showed that the recombinant enzyme had a molecular of 76 ku band which was consistent with the predicted molecular mass, and it had the ability to catalyze the conversion of maltose into trehalose. The recombinant pPICZaA-TreS plasmid successfully constructed and integrated into Pichia pastoris genome and expressed as expected.
    4  Physicochemical Properties of Acid-soluble Collagen from Skin of Clarias fuscus
    LI Ba-fang GUO Ming HOU Hu WANG Shan-shan
    2013, 29(11):2580-2585.
    [Abstract](1488) [HTML](0) [PDF 552.49 K](793)
    Abstract:
    Fish skin of Clarias fuscus was a by-product of freshwater aquatic products processing, which is rich in resources. To improve the utilization of aquatic by-products, the fish skin of Clarias fuscus was used as raw materials for acid-soluble collagen (ASC) extraction at 4 ℃. It’s the physicochemical of the extracts was then studied. The hydroxyproline experiment showed that the extraction yield of ASC reached 20.32%. The sample met the characteristics of type I collagen with the most abundant amino acid of glycine (32.59%) by analysis of amino acid compositions. SDS-PAGE and ultraviolet-visible (UV-Vis) spectra identified the sample was of a type I collagen protein with high purity. Fourier transform infrared (FT-IR) spectra of ASC suggested that the protein presented in the triple helix structure. The denature temperature (Td, 26 ℃) and shrinkage temperature (Ts, 53.01 ℃) showed that the thermal stability of the collagen was lower than collagen from mammalian. X-ray diffraction diagram showed the internal structure of the collagen molecules from Skin of Clarias fuscus.
    5  Acceleration of Hard Cheese Ripening at Elevated Temperature
    ZHAO Zheng YANG Wei XU Yao ZHANG Dong-jing ZHAO Yan-xing
    2013, 29(11):2586-2590.
    [Abstract](1007) [HTML](0) [PDF 617.58 K](647)
    Abstract:
    The mature characteristics of hard cheese ripening in 90 days separately at 4 ℃, 10 ℃, 20 ℃ were studied, and the change of soluble nitrogen content, microstructure, pH, free fatty acids, volatile compounds, sensory characteristics were investigated. The results demonstrated that the contents of soluble nitrogen, free fatty acids and volatile compounds were significantly increased by increasing temperature. Compared with hard cheese ripened at 4 ℃ for 90 days, the hard cheese ripened at 10 ℃ for 60 days and 20 ℃ for 30 days owned the same or more soluble nitrogen, free fatty acids and volatile compounds; furthermore, tiny difference of microstructures were found among the three conditions. Although 20 ℃ could accelerate hard cheese ripening to some extent, bad effects were found on volatile compounds and texture of the cheese. Therefore the best suitable temperature for elevating hard cheese ripening was 10 ℃.
    6  Decomposition Kinetics of Allicin in Pentane and Ethanol
    ZHOU Hua LI Wen-qing YAN Ri-an HUANG Xue-song OU Shi-yi
    2013, 29(11):2591-2594.
    [Abstract](1044) [HTML](0) [PDF 469.64 K](593)
    Abstract:
    The kinetic parameters for decomposition of allicin in pentane or ethanol were determined. The activation energies and pre-exponential factors were separately 56.0 kJ/mol and 9.2×105 in pentane as well as 72.0 kJ/mol and 1.5×106 in ethanol. The intermolecular interaction energy between allicin and solvent was calculated by density functional theory (DFT) at the B3LYP/6-31+G(d+P) leve1. The results showed that the intermolecular interaction energy between allicin and ethanol (31.18 kJ/mol) was higher than that between allicin and pentane (1.12 kJ/mol). Natural orbital theory (NBO) analysis showed that the anti-bonding orbital stabilization energy between the lone pairs of O (13) in allicin and hydroxyl (O(21)-H(20)) in ethanol molecule was 45.45 kJ/mol, which illustrated that strong hydrogen bonding interaction between them dispersed the negative charges in O atom of allicin. Therefore it became more difficult in the intramolecular to transfer H atom during decomposition of allicin in ethanol.
    7  Effect of Vacuum Pickling on Salt Osmotic Dehydration and Quality Changes of Pork
    ZHANG Li-yan XIONG Ling
    2013, 29(11):2595-2600.
    [Abstract](1370) [HTML](0) [PDF 450.37 K](845)
    Abstract:
    The influence of vacuum pressure and brining time on salt osmotic dehydration and quality changes of pork in vacuum pickling process were studied. The results indicated that, as the vacuum pressure and curing time increased, the quantity of salt endosmosis increased exponentially, while water content decreased and reached the equilibrium at about 6 h. The mass of pork kept rose in the vacuum brining process, and the mass-gain rate was increased significantly as the vacuum pressure increasing. Salt penetration rate decreased and the constant of salt penetration rate increased first and then decreased with the vacuum pressure increased, and reached its maximum value of 0.977 at 0.08 MPa. Vacuum pressure had slight impact on pH of the pork. Soluble protein content in salt solution had the similar change as the constant of salt penetration, which showed the highest value at 0.08 MPa. The values of brightness (L*) and yellowness (b*) were enhanced while redness (a*) reduced during the salting process. Vacuum brining could improve the tenderness of pork obviously, and the hardness, elasticity and chewiness were enhanced gradually. The resilience decreased and cohesiveness decreased after the first increases.
    8  Influence of Apple Polyphenols on the Properties of Polyphenol Oxidase
    YI Jian-hua LI Mei-li ZHU Zhen-bao
    2013, 29(11):2601-2606.
    [Abstract](1316) [HTML](0) [PDF 741.06 K](771)
    Abstract:
    Influence of different apple polyphenols on the properties of polyphenol oxidase (PPO) was investigated and the suitable conditions of PPO enzymatic oxidation were obtained by employing chlorogenic acid, catechin, epicatechin and phlorizin as substrates and by determining the change of absorbance in the phosphate buffer model system. The results showed that the substrates had an important impact on the reactive characteristics of PPO. The optimum pH of enzymatic oxidation of chlorogenic acid, catechin, epicatechin and phlorizin by PPO were 5.0, 4.0, 4.5 and 4.5, respectively. The best temperature of enzymatic oxidation of the above-mentioned substrates were 55, 70, 60 and 50 ℃, respectively. And the polyphenol substrate concentration for the four substrates were 5, 0.5, 2 and 10 mM, respectively. Furthermore, appropriate increase of enzyme activity could promote the enzymatic browning reaction. The affinity of the four substrates for PPO was in the order of catechin ? epicatechin﹥chlorogenic acid ? phlorizin. This study will supply a basis for browning control of apple juice.
    9  Correlation Analysis between Textural and Physicochemical Properties of Simiao Rice
    ZHONG Guo-cai CHEN Wei CHEN Jia-dong ZENG Lin WEN Yu-ke HUANG Zhi-liang WAN Juan LAO Chuan-zhong
    2013, 29(11):2607-2611.
    [Abstract](1531) [HTML](0) [PDF 474.85 K](651)
    Abstract:
    Seven kinds of raw Simao rice samples from Zengcheng were selected to investigate the correlations among textural properties of cooked rice, thermal characteristics of rice flour, gelatinization, and physicochemical properties of rice. The results showed that, hardness and adhesiveness of these samples varied greatly, but their cohesiveness, gumminess, chewiness and resilience were similar to each other. There were significant relationships between amylose content of rice and textural characteristics of cooked rice. Hardness and adhesiveness of cooked rice, showed negative correlation with the enthalpy, but positive correlation with onset temperature of rice starch gelatinization. Furthermore, hardness, adhesiveness, cohesiveness, gumminess, chewiness and resilience of cooked rice possessed negative correlation with final viscosity of gelatinization and breakdown value of rice starch, but positively correlated with the setback value. Meanwhile, viscosity had negative correlation with consistence value of rice starch. This reaserch will be useful for development and control of the quality and stability of rice formulations.
    10  An Empirical Model for Predicting Conversion of the Synthesis of n-Butyl Lactate by Lipase-Catalyzed Esterification
    ZHONG Jin-feng QIN Xiao-li WANG Yong-hua
    2013, 29(11):2612-2615.
    [Abstract](1355) [HTML](0) [PDF 471.75 K](615)
    Abstract:
    Butyl lactate is an important green solvent, which was widely used in food and cosmetic industries. Enzymatic process for the synthesis of butyl lactate provides high-efficient manufacture. An empirical model for predicting conversion of the synthesis of n-butyl lactate by lipase-catalyzed esterification was proposed based on the relationships of reaction time, reaction temperature and lipase concentration in a given n-butyl alcohol/lactic acid molar ratio (1.5:1). The model of Er, % = 0.168×D0.018×(T-7.5) ×Ln(1+100t) was established, with a good correlation (R2 = 0.995) between the experimental data and predicting data. Such data would be valuable for the process control and optimization during the synthesis of n-butyl lactate.
    11  Changes of Main Microflora in Changde Fresh Wet Rice Noodles
    TONG Li-tao ZHOU Su-mei LIN Li-zhong ZHONG Kui LIU Li-ya ZHOU Xian-rong LIU Ye-ji
    2013, 29(11):2616-2620.
    [Abstract](1525) [HTML](0) [PDF 517.64 K](670)
    Abstract:
    To explore the changes of microflora in the natural fermentation process of Changde fresh wet rice noodles, the main microbial species, variation and pathogen threats were studied. The results showed that the lactobacillus and yeast were dominant bacteria during the natural fermentation. The pH of fermentation broth was decreased from 6.21 to 3.69 after 8~12 h fermentation, and then increased slightly to 4.20 at the ends of fermentation. Compared with unfermented rice, the contents of proteins, crude fat and ash in fermented rice were significantly decreased by 24.92%, 31.01%, and 81.37%, respectively. The starch content had no obvious change. The injection of Bacillus cereus presented less effect to pH of the fermentation broth. The risk of Bacillus cereus at 103cfu/g level in fermentation broth was controllable for its number was gradually reduced with fermentation forward. However, when it reached 104 cfu/g, Changde fresh wet rice noodles had a security risk, although its number was decreased after fermentation.
    12  Quality Change and Shelf-Life Prediction of Chilled Pork during Storage
    U Hai-ning LI Qiang LI Wen-zhao ZHANG Zhi-gang GUO Shuang CHENG Chen
    2013, 29(11):2621-2626.
    [Abstract](1450) [HTML](0) [PDF 550.24 K](971)
    Abstract:
    Changes of chilled pork in quality were studied at different storage temperatures. Factor analysis and Q10 model were applied to establish shelf life prediction model of chilled pork. Sensory quality, aerobic bacterial count (ABC), TVB-N, TBARS, pH and color(L*, a*, b*) were evaluated as quality indicators at 0, 5, 10, 15 and 20 ℃. By analysis of Pearson product-moment correlation, the limited value of the physiochemical factors would be got by sensory evaluation at the end of shelf-life, in order to conduct shelf life prediction model. The results showed that, the ABC, TVB-N, pH, TBA, and color (b*) increased with prolonged storage time. The increase of storage temperature resulted in significant increases of these factors. The sensory quality and color (a*) decreased with prolonged storage time and dropped promptly with increasing storage temperature. Theverification test of chilled pork stored under 4 and 12 ℃ showed that, the relative error was within ±10%. It means that the model can be used to predict the quality of chilled pork satisfactorily.
    13  The Degradation Pathway of Nitrite by LCR 6013 and the Primary Localization of Its Nitrite Reductase
    ZHANG Xin-yue LIU Dong-mei XU Xi-lin LI Ping LI Li
    2013, 29(11):2627-2632.
    [Abstract](1317) [HTML](0) [PDF 554.73 K](703)
    Abstract:
    The effects of Vc, NaCl on the nitrites degradation by Lactobacillus casei subsp. rhamnosus 6013 (LCR 6013) in the De Man, Rogosa and Sharpe medium (MRS) were investigated. The degradation pathway was confirmed by electron capture gas-chromatography and indophenol blue colorimetric method. The nitrite reductase (NiR) localization was examined by measuring the enzyme activity of different cellular components from LCR 6013 cells. In MRS, the degraded nitrites concentration reached the highest to 9.29 μg/mL and 9.89 μg/mL when NaCl and Vc concentrations were 0.75 % and 0.02 %, respectively. To compare with the control, the degradation effect of treatment was significant (p?0.01). When the initial concentration of nitrites was 10.00 μg/mL, the degradation products contained 28.81?10-6 N2O. The strain LCR 6013 completely degraded the nitrites with initial concentration of 50.00 μg/mL after 16 h incubation And the volume fraction of N2O reached 96.61?10-6 after 14 h. The enzyme activity of nitrites from the periplasm extraction was 2.5 fold higher than that from the cytoplasm extraction. In summary, the effective degradation of nitrites is due to the reaction of the nitrite reductase in the LCR 6013 cell. The most likely pathway of degradation was as follows: NO2-?NO?N2O?N2, rather than ammonification. The degradation products contain N2O gas.
    14  Antioxidant and Antiproliferative Activities of 5-Hydroxymethylfurfural
    ZHAO Ling CHEN Jian-ping LI Lin ZHOU Rong SU Jian-yu
    2013, 29(11):2628-2642.
    [Abstract](1591) [HTML](0) [PDF 521.27 K](607)
    Abstract:
    The antioxidant of 5-Hydroxymethylfurfural (5-HMF) was evaluated by ABTS, DPPH and hemolysis assay. In the hemolysis assay, the morphological changes of erythrocytes were observed directly by an environmental scanning electron microscope (ESEM). Meanwhile, MTT assay was carried out to investigate antiproliferative activities of 5-HMF. The results showed that the scavenging rates of ABTS and DPPH were 53.98 ± 0.016% and 17.80 ± 0.010%, respectively when using 5-HMF at 6.4 mM. And the emolysis inhibition rate reached 89.95 ± 0.001% at a concentration of 12.0 mM, suggesting that 5-HMF could inhibit the hemolysis oxidative damage induced by AAPH. The results was verified by observation of the morphological changes of erythrocytes using ESEM. 5-HMF showed a higher antiproliferative activity on human melanoma A375 cells than L02 cells and SW480 cells, which was certificated by inverted microscope.
    15  Malus micromalus Makino Polyphenols and Its Antioxidant Activity
    WANG Meng WANG Min LI Huan-yu WANG Yong WU He-ping
    2013, 29(11):2633-2637.
    [Abstract](1466) [HTML](0) [PDF 509.28 K](801)
    Abstract:
    To explore the antioxidant activity and the polyphenols of Malus micromalus Makino, the ultrasonic method was utilized to extract the antioxidants from different parts of the fruit, their total phenolic (TP), total flavonoid (TF) contents, phenolic compounds and antioxidant activities were analyzed. The antioxidative capacity of the extracts was evaluated by reducing power, DPPH?and ABTS?+ scavenging, while HPLC was used to investigate the composition of polyphenols. The contents of TP and TF in denucleated Malus micromalus Makino (DM) were 60±5.03 mg galic acid q/100 g FW and 54.45±1.52 mg rutin eq/100 g FW, respectively, being significant higher than those in peel and pulp of the fruit. There were obvious positive correlations between TF content and the three antioxidant activity indices. And TP content had less positive correlations with the three indices. Hydroxybenzoic acids including vanillic acid, gallic acid were the main polyphenols of Malus micromalus Makino. In addition, flavonols gained the highest contents in peels. And catechin and epicatechin were only detected in pulp. In all detected substances, the content of rutin in peels was the highest with 819.88±10.17 μg/100 g. Moreover, chlorogenic acid was also an important phenolic compound in Malus micromalus Makino.
    16  Effects of Enzymatic Hydrolysis with Cell Wall Proteinase (CEP) on Structural and Functional Properties of Casein
    REN Xiao-fen PAN Dao-dong ZENG Xiao-qun CAO Jin-xuan SUN Yang-ying
    2013, 29(11):2643-2648.
    [Abstract](1056) [HTML](0) [PDF 575.62 K](648)
    Abstract:
    The structural characteristics of casein hydrolyzed by cell wall proteinase (CEP) from Lactobacillus acidophilus were analyzed by granularity and Fourier transform infrared spectroscopy (FTIR). The effects of different degree of hydrolysis (DH 2.4%, 4.5%, 7.1% and 8.3%) on functional properties of casein were determined by emulsibility, emulsion stability, solubility, antioxidant activity and ACE inhibitory activity. The results of FTIR spectra indicated remarkable changes in the chemical compositions and macromolecular conformation of casein after hydrolysis. The secondary structure of casein was changed with the enzymatic treatment. The nanoparticle size analysis showed that the casein particles decreased during the initial hydrolysis time (DH<4.5%), but increased for further hydrolysis, which resulted in the highest emulsion stability under DH 4.5%. The emulsibility and solubility of casein first increased and reached the highest when DH was 7.1%, and then decreased with the further hydrolysis. In addition, the casein hydrolysates had certain ACE inhibitory activity and antioxidant properties, and the DPPH removal capacity within a certain range increased as DH and concentration increased. The highest DPPH removal capacity was 35.00% with DH 8.3% and hydrolysates concentration of 5 mg/mL. Therefore, the enzymatic hydrolysis with CEP can effectively improve the structural and functional properties of casein. This research will provide useful theoretical basis for the production of ACE inhibitory peptides derived from casein.
    17  Effect of Smilax Glabra Formulated Preparation on the Hyperuricemia Induced by Potassium Oxonate
    CHEN Lu-ying YANG Ji-guo QIU Xiao-bin TAN Kai-yuan WEI Qing-yi YUAN Er-dong NING Zheng-xiang
    2013, 29(11):2649-2652.
    [Abstract](1369) [HTML](0) [PDF 475.53 K](770)
    Abstract:
    The rat model of hyperuricemia was established by oral gavage with potassium oxonate, and the effect of a Smilax Glabra prescription on the hyperuricemia was studied. The rat model of hyperuricemia was established in accordance with 1.5 g/(kg?d) continuous intragastric dose by potassium oxonate for 30 d. When tested at the 15th and 30th d, serum uric acids in the model were significantly different to the normal control group, urea nitrogen and creatinine in the serum had no pathological change. After continuously oral gavage for 30 d with Smilax Glabra prescription (15th and 30th d tests), the serum uric acid and urea nitrogen were reduced in the corresponding model with different doses and the creatinine was significantly reduced with high doses. Therefore the Smilax Glabra prescription has a certain effect on potassium oxonate-induced hyperuricemia.
    18  Effect of Fractionation of Maillard Reaction Products in Glucose-lysine Model System on Inhibitory Properties of Banana Enzymatic Browning
    ZHOU Ya ZHANG Hai-de LI Yi-Xing LI Fen-fang YUAN De-bao ZHENG Xiao-yan TAN Lin CHEN Jiao MA Wei-hong JIN Zhi-qiang
    2013, 29(11):2653-2657.
    [Abstract](1320) [HTML](0) [PDF 506.31 K](646)
    Abstract:
    Screening of non-toxic and non-sulfur anti-browning inhibitors is a hot topic. Maillard reaction products (MRPs) in fructose-lysine model system exhibited strong inhibitory effect on banana enzymatic browning in our previous study. The study mainly discussed the effect of fractionation of the products on the inhibition propertie. Ultrafiltration and dialysis were applied to divide the MRPs into three grades: MW >5000 u fraction, MW < 3500 u fraction and 3500 u < MW < 5000 u fraction. The antioxidant activities (including DPPH?scavenging ability and reducing power), chelating copper activity and the inhibitory effect on banana polyphenol oxidase were evaluated. The fraction with MW >5000 u showed the best chelating copper ion ability, followed by that with MW < 3500u. The fraction with MW <3500 u exhibited the best DPPH?scavenging ability (46.13%) and polyphenol oxidase inhibitory ability (IC50 0.089 mg/mL). Free enzyme inhibition constant (KI) and enzyme-substrate complex inhibition constant (KIs) of the obove-mentioned three parts were KI=0.297 mmol/L and KIS=1.744 mmol/L (MW >5000 u), KI =0.212 mmol/L and KIS=1.656 mmol/L (5000 u > MW >3500 u), KI =0.205 mmol/L and KIS=1.536 mmol/L (MW <3500 u). The fraction with MW <3500 u could combine free enzyme and enzyme-substrate more tightly. In conclusion, the fraction with MW <3500 u was shown to be the best for inhibiting banana enzymatic browning.
    19  Screening and Identification of a Xylanase-producing Strain during Tobacco Fermentation
    FAN Yi-wen LIN Mei-yan LI Xu-dong LI Zhe CHEN Ya-wen HAN Shuang-yan
    2013, 29(11):2658-2662.
    [Abstract](1268) [HTML](0) [PDF 503.17 K](625)
    Abstract:
    Microorganisms play an important role in tobacco aroma improving. The tobacco samples were selected from five areas (Bijie, Chuxiong, Kunming, Qujing, and Xiangxian) of three years (2007, 2008 and 2009), using nutrient agar for purification, and then cultured in the screening xylanase base for rescreening. The xylanase-producing strains was screened by determining the appear transparent circles among the colonies after Congo red staining. The xylanase activities of these strains were measured by 3,5-Dinitrosalicylicacid (DNS). Qb4 strain, which had higher xylanase activity, were identified as Bacillus methylotrophilus by analysis of its morphological, physiological and biochemical characteristics, carbon source utilization and the 16S rRNA conserved sequence.
    20  Physicochemical Properties and Flavor components of Low-Salt Pickle Inoculated with Lactic Acid Bacteria
    GAO Shi-yang SUN Zhi-dong DU Xin-yong HE Guo-qing
    2013, 29(11):2663-2668.
    [Abstract](1458) [HTML](0) [PDF 577.61 K](850)
    Abstract:
    In order to get a more healthy and safe pickle, lactic acid bacteria (LAB) was inoculated in the pickle-preparation process under low salt condition. The pH, the number of LAB, and the nitrite content in the pickle progress, as well as the amino nitrogen content and the flavor components of the final products were determined when adopting different processing methods including one inoculation, multiple inoculation and the traditional high salt pickling process. The results showed that, at primary pickle stage, pH values of the pickle inoculated only once and inoculated by several times quickly reduced to 4 or less than 4, and the number of lactic acid bacteria reached to 108 cfu/mL. The nitrite peak value (1 μg/mL) appeared 4~6 days earlier than that of the control. During the repeat pickle process, the pickle inoculated by several times showed a contant pH range of 3.6~3.8, stable number of LAB and the lower nitrite peak value below 0.3 μg/mL at the post period. The pickle inoculated with LAB had more amino nitrogen content and flavor components than the control group, and multiple inoculation process was better than one inoculation for achieving better quality of the product.
    21  Synthesis of 3-Pyridinecarboxylic Acid Leaf Alcohol Esters and its Application in Cigarette
    ZHAO Ming-qin BAO Xiao-rong JI Xiao-ming LIU Hui-jie ZHANG Huan-huan
    2013, 29(11):2669-2674.
    [Abstract](1397) [HTML](0) [PDF 649.10 K](736)
    Abstract:
    In order to develop new cigarette flavors, synthetic novel pyridine compounds were added to cigarettes to supplement the aroma, and improve the smoking quality of cigarette. 3-Pyridine carboxylic acid was used as a raw material and 3-pyridine carbonyl chloride hydrochloride was obtained through acylation reaction with thionyl chloride. Then the same volume of leaf alcohol was added, pyridine as solvent and catalyst, and reacted for 12 h at 6 0 ℃ to synthetize 3-pyridine carboxylic acid leaf alcohol ester. The structure of the compound was confirmed by 1H NMR, 13C NMR, IR and HRMS spectra. And then its thermal stabilities and cracking behaviors at 300 ℃, 600 ℃ and 900 ℃ were studied. Fifty kinds of pyrolysis products were authenticated and most of them were aroma components. The smoke panel test showed that 3-pyridine carboxylic acid leaf alcohol ester could improve the aroma quality, increase the aroma quantity, harmonize the aroma, clean the after-taste and reduce offensive odor of cigarettes. At concentration of 1.50‰, it showed the most appropriate flavoring effect.
    22  Preparation and Characterization of PVDF Microporous Membrane via Thermally Induced Phase Separation Method
    XIAO Kai-jun TANG Xu DONG Hao XIONG Pu
    2013, 29(11):2675-2680.
    [Abstract](1383) [HTML](0) [PDF 572.60 K](646)
    Abstract:
    Polyvinylidene fluoride (PVDF) microporous membrane was prepared using PVDF as raw material and dimethyl phthalate as diluents through thermally induced phase separation (TIPS) method. The properties and thermodynamic features of PVDF microporous membrane were characterized by scanning electron microscope (SEM), differential scanning calorimetry (DSC) and tensile strength test. The results indicated that DMP had an excellent compatibility with PVDF. When the DMP contents increased from 50% to 80%, the pure water flux and the porosity of PVDF microporous membrane improved from 65 L/(m2?h) and 34% to 230 L/(m2?h) and 77%. While the breaking tensile strength and breaking elongation of the microporous membrane decreased gradually along with the increase of DMP content. Moreover, the pure water flux of PVDF membrane improved by 3~4 times in dealing with organic solvents. Meanwhile, thermodynamic study showed that with the DMP contents increased, PVDF crystal grain became more and more fuzzy and the cavity between spherocrystal became bigger, and the spherocrystal size presented a descendant tendency.
    23  Development of a Synthesized Test Platform of the Axial Motion and the Turnover Motion of Eggs on Convey Rollers
    JIANG Song JIANG Yi-yi SUN Ke WANG Guo-jiang CHEN Shu-lai CHEN Zhang-yao
    2013, 29(11):2681-2685.
    [Abstract](1146) [HTML](0) [PDF 647.68 K](587)
    Abstract:
    In order to clarify the mechanisms of the axial motion and the turnover motion, optimize the structure and conveying parameters of the device of automatic orientation of eggs, a synthesized test platform was developed. The structure and conveying parameters(the diameter, material and surface situation of rollers, the rotate speed and convey speed of rollers, the roller center distance, the guide rod bending angle and the distance between roller and guide rod) could be adjusted. The processes of the axial motion and the turnover motion of eggs were recorded by high definition digital camera. Then, the motion and attitude parameters of eggs were analyzed by imagines processing software. The verification test with thirty eggs showed that the laws of the axial displacement and the deflection angle as well as the turn angle of the turnover motion tested by the synthesized test platform were accordance with that tested by the device of automatic orientation of eggs. During a period of running, the synthesized test platform remained stable, operational convenient and the results of tests were reliable.
    24  Mutation and Screening of Neutral Protease High-yield Strain
    ZHU Ming-jun OU Jian-fa CHEN Hong-tu
    2013, 29(11):2686-2691.
    [Abstract](1311) [HTML](0) [PDF 550.91 K](637)
    Abstract:
    The biological effects of physical and chemical mutagenesis inducements on neutral protease produced by Bacillus subtilis SHB 2010-1 were investigated. Six selective media were studied on screening the efficiency of high-yield strain. Skim milk agar was proved to be the excellent cell culture medium, with which the hydrolysis zone was easily observed. On the base of the corresponding death curves, death rate at 90-95% was selected to take two-round ultraviolet-diethyl sulfate mutation. The enzyme activities of mutant strains from each step were 1.49%, 10.99%, 38.77% and 59.68%,respectively, higher than the original strain. The results showed that complex mutagenesis performed a more significant effect than single factor. The mutant strain named DES-59 were finally screened with the maximum enzyme activity of 7307 U/mL in 50 mL fermentation medium after 60 h, and the cell density of 2.205×1010 CFU/mL. The nprE gene of the mutant DES-59 was the same as sequence in database, indicating that the improvement was not contributed by the structural modification of neutral protease A, but by some unstated sites.
    25  Hot-air Drying Characteristics and Kinetics Model of Pleurotus eryngii
    CHEN Jian-kai LIN He-tong LI Hui TAN Hui-jun LIN Yi-fen
    2013, 29(11):2692-2699.
    [Abstract](1447) [HTML](0) [PDF 664.34 K](760)
    Abstract:
    The hot-air drying characteristics of Pleurotus eryngii were studied in terms of different hot-air temperatures, air velocities, material size and accumulation layers of material, meanwhile a hot-air drying mathematical model was established. The test showed that these factors significantly affected the hot-air drying characteristics. Increasing the air temperature and velocity, or lowering size material, single accumulation layer could fasten the drying speed and shorten drying time of Pleurotus eryngii. The speed of a hot-air drying would be faster at air temperature of 80 ℃ with the material size of 1 cm × 1 cm, or single accumulation layer. When air velocity was 1.5 m/s, the drying speed increased as the drying time was shorted. The experimental drying data of Pleurotus eryngii was used to fit five available drying models by applying Matlab 7.0 and using Gauss-Newton algorithm, and the coefficients of models were determined by non-linear regression analysis. It was showed that Two-term model had higher correction coefficient (R2), lower square sum of error value (SSE) and root mean square error value (RMSE), which could exactly describe and predict the moisture dynamics of Pleurotus eryngii during hot-air drying process.
    26  Effect of Different Color Protection Technologies on Color and Fatty Acid Compositions of Chestnut Kernels
    YANG Fang ZHANG Cong-lan WANG Tie-dan DU Ping LI Qing ZHANG Yan
    2013, 29(11):2700-2705.
    [Abstract](1105) [HTML](0) [PDF 650.64 K](675)
    Abstract:
    Polyphenol oxidase (PPO) and peroxidase (POD) are two main factors that lead to browning in Castanea mollissima. Solvent soaking was used to inhibit enzyme activity and protect the color of chestnut kernel. On the basis of the stability studies of PPO and POD, the parameters of temperature, time and pH were determined. Effects of soaking color protection technology on the total color difference of chestnut kernel (△E *) and fatty acid composition were also studied. The results showed that when pH was less than 4.0, the PPO and POD activities in the chestnut kernel were lower, and the total color difference was less. PPO activity was significantly decreased after the soaking treatment for 10 min at 30 ℃, while POD activity reached the lowest after the treatment for 25 min at 30 ℃. The optimum conditions for soak color protection process were pH 3.8, temperature 25 ℃, and time 20 min. After this color-protecting treatment, the unsaturated fatty acid content of chestnut lipid decreased slightly, while the major fatty acid (linoleic acid) content remained the same. The color protection technology had little significant effect on the fatty acid composition and flavor of the chestnut and could be used as an effective method of protecting the color of chestnut
    27  Method Optimization of Determining Quercetin Content in Mulberry Leaf by Fluorescence Spectrophotometry
    XU Yan-yang LI Ke-jing JIA Hong-lei
    2013, 29(11):2706-2711.
    [Abstract](1109) [HTML](0) [PDF 550.29 K](635)
    Abstract:
    To explore a new method for determining quercetin content in mulberry leaf, fluorescence spectrometry was used and corresponding operational parameters were discussed. The effects of ethanol volume fraction, static time, static temperature, addition of 5% Al(NO3)3 solution and pH value on fluorescence intensity of quercetin were studied with quercetin standard as reference. On the basis of single-factor experiments, significance analysis and interaction effects of various factors were optimized by response surface methodology. The results indicated that optimal detection conditions of quercetin content in mulberry leaf were ethanol concentration 100%, static time 40 min and static temperature 13 ℃. Quercetin content in mulberry leaf was measured of (41.92±0.11) mg/g and the relative standard deviation was 0.34% under above conditions. Established linear regression equation of quercetin standard curve was y=240.14x+7.0952, and the correlation coefficient was R2=0.9950 with a linear range of 0~1.5×10-2 mg/mL. This method was suitable for detection of quercetin content in mulberry leaf.
    28  Preparation and Selection of Polyclonal Antibodies against Malachite Green
    SUN Jing-wei HU Zhang-li
    2013, 29(11):2712-2716.
    [Abstract](1427) [HTML](0) [PDF 549.41 K](921)
    Abstract:
    Three haptens for malachite green were designed and synthesized. Then the haptens were separately coupled to keyhole limpet hemocyanin (KLH) as immunogens and coupled to ovalbumin (OVA) as coating proteins by using active ester method. New Zealand white rabbits were immunized with the three immunogens, and all produced high titer antisera antibodies angainst malachite green. All the antibodies and the coating proteins were screened and then the optimal antibody-coated antigen combination was obtained. The selected polyclonal antiserum was purified by Sepharose FF-Proteins A affinity chromatography. The titer and characteristic were determined by indirect ELISA. The results showed that the antibody titer and IC50 reached 1:12000, and 0.65 ng/mL, respectively. And the antibody had good specificity. The cross-reactivity was less than 10% for leucomalachite green and leucocrystal violet but the antibody exhibited significant cross reactivity with crystal violet (18.73%). The study provided basis for rapid detecting malachite green residues in food by immune analysis.
    29  Separation and Purification of Flavonoids Rrom Rhizoma Smilacis Glabrae by β-cyclodextrin-Starch Resin Polymer
    ZHANG Qing-feng CHEN Ji-guang SHANGGUAN Xin-cheng ZHENG Guo-dong YIN Zhong-ping
    2013, 29(11):2717-2721.
    [Abstract](1279) [HTML](0) [PDF 612.07 K](601)
    Abstract:
    Three water insoluble cyclodextrin (CD)-starch copolymers (α-, β-, and γ-CDP) were synthesized by using epichlorohydrin as the crosslink reagent. Comparative study of the kinetic adsorptions of α-, β-, and γ-CDP to astilbin showed that β-CDP had the bestabsorption property. Static equilibrium adsorption studies revealed that β-CDP adsorption capacity rose with the increase of equilibrium concentration of astilbin.And its adsorption capacity decreased with the increase of temperature, which revealed that the adsorption was an exothermic process. Under the conditions of 4 ℃ and equilibrium concentration 360 μg/mL, adsorption capacity of β-CDP to astilbin was 49.52 mg/g. The adsorption could quite fit to the Freundlich adsorption isotherm, indicating that it was the nonuniform, multilayer adsorption. About 91.97% of adsorbed astilbin could be recovered by use of 60% ethanol as the elution solvent. The yield of flavonoids was 2.28% when β-CDP was used for separation and purification of flavonoids from Rhizoma Smilacis Glabrae. The contents of astilbin and total flavonoids were 51.71% and 76.63%, respectively, as determined by HPLC.
    30  Effects of Micronization Methods on Physicochemical Properties of Pleurotus eryngii Powders
    LIU Su-wen CHANG Xue-dong LI Hang-hang GAO Hai-sheng
    2013, 29(11):2722-2727.
    [Abstract](1430) [HTML](0) [PDF 574.26 K](615)
    Abstract:
    The effects of micronization methods, such as shear pulverization, mechanical milling and jet milling, on the physicochemical properties of Pleurotus eryngii powders were investigated. Six powders from dried mushroom cap and stipe by the three grinding methods were prepared. Compared with shear pulverization and mechanical milling, jet milling effectively reduced particle size (cap14.16 μm and stipe 13.16 μm) and brought about a narrow and uniform particle size distribution. Cap powders possessed higher protein (18.34 g/100 g, dry basis), fat and ash contents. With the same material (cap or stipe), powders from jet milling had higher values in specific surface area, bulk density, fluidity, water soluble index and solubility of protein and polysaccharide (p<0.05), but lower values in the water holding and swelling capacities than other two processed powders. With the same grinding method, cap powders owned higher values in water soluble index, swelling capacity, bulk density, and solubility of polysaccharide than stipe powders. Under the same humidity environment, the water activity (Aw) of jet milling powder was less than the mechanical milling and shear pulverization powder, and aw of cap was less than that of stipe. The Oswin model was considered as the optimal regression equation of moisture sorption isotherms within different kinds of Pleurotus eryngii powders at 25 ℃, with the highest value of regression coefficient (0.9902~0.9943) and lowest value of relative errors.
    31  Optimization of the Gutting Process of Trash Fish
    HU Xiao-liang CHEN Qing-yu SHEN Jian
    2013, 29(11):2728-2734.
    [Abstract](1192) [HTML](0) [PDF 571.37 K](663)
    Abstract:
    A self-developed gutted mechanism were employed for cutting Trachurus japonicus and Synodus macrops to avoid fish damage and increase gut removal rate in gutted process. The relationships of acceptability evaluation with transmission speed, cut knife rotation speed and gutted wheel rotation speed were studied by quadratic regression rotational combination design, and the mathematical model between the acceptability evaluation and operating parameters was established. The best parameters of gutted process of Trachurus japonicus were: transmission speed 1.92 m/s, cut knife rotation speed 1154.0 r/min,and gutted wheel rotation speed 60.0 r/min. And the optimized parameters for Synodus macrops were: transmission speed 1.39 m/s, cut knife rotation speed 1280.0 r/min, and gutted wheel rotation speed 95.0 r/min. Under these conditions the maximum value for acceptability evaluation of gutted effect was achieved.
    32  Preparation of Water-soluble Curdlan Oligose and Determination of Its Activity
    WANG Ying-jie ZHAO Shuang-zhi SU Li LAN Wen-zhong
    2013, 29(11):2735-2741.
    [Abstract](1230) [HTML](0) [PDF 572.52 K](606)
    Abstract:
    Soluble curdlan oligose with low molecule weight (CRDO) was prepared from curdlan by oxidative degradation of hydrogen peroxide in alkaline homogeneous phase. The effects of NaOH concentration, dosages of H2O2,temperature,and time on curdlan degradation were studied. The optimal hydrolysis conditions were determined as follows: NaOH concentration 2 mol/L, H2O2 dosage 1.5% (V/V), temperature 40 ℃ and reaction time 48 h. The degradative production was examined by paper chromatography and infrared spectrum analysis, which demonstrated that the chemical structure of the hydrolysates had no change. .In addition, its antitumor activity antibacterial activity and effects on growth performance of Penaeus japonicus were studied. The results showed that it could inhibit the growth of S-180 sarcoma. When CRDO dosage was 50 mg/kg of a rat, the antitumor acitivity was 48.73%. Meanwhile, it could improve the survival rate of Penaeus japonicas, and make the PO activity up to the highest of 2.96 U/mg when CRDO dosage was 400 mg/kg of feed. The antimicrobial tests indicated that the water-soluble CRDO had certain antibacterial effect on staphylococcus aureus and had weak antimicrobial effect on E. coli, but no inhibition on angel yeast.
    33  Improved Method for High-performance Liquid Chromatography Assay of Angiotensin I Converting Enzyme Inhibitory Activity
    ZHAO Mou-ming LAI Chen-rong CUI Chun
    2013, 29(11):2742-2746.
    [Abstract](1290) [HTML](0) [PDF 608.70 K](765)
    Abstract:
    A modified method for determining the inhibitory activity of angiotensin-converting enzyme (ACE) from Pinctada martensi hydrolysate by HPLC was proposed. This method eliminated the effect of absorption peaks of some samples at the retention time of hippuric acid (HA) at 228 nm, and improved the measurement accuracy. The interfering peak area of the hydrolyzate decreased with increasing hydrolysis time. The interfering peak area of the hydrolysate by endogenous enzymes decreased to zero after 24 hours. The effects of different adding sequences of N-Hippuryl-His-Leu hydrate (HHL) and ACE on the assay were compared. Results showed that ACE inhibitory activity when HHL was added firstly was higher than those when ACE was added firstly. Therefore, in determination of ACE inhibitory activity, the same addition sequence of ACE and HHL should be adopted.
    34  A Rapid Method of Biological Chip for Detecting Clenbuterol Based on Surface Plasmon Resonance
    LI Ying QI Pan MA Xiao ZHONG Jin-gang CHEN Jiang-han ZHANG Guan-wen
    2013, 29(11):2747-2751.
    [Abstract](1379) [HTML](0) [PDF 538.03 K](662)
    Abstract:
    Clenbuterol Hydrochloride, categorized as brown meat essence, is prohibited in China. Clenbuterol is detected by two methods of continuous and rapid according to the law of immune response. The methods are based on surface plasmon resonance by using the biological chip. They are capable of detecting anti-Clenbuterol without labeling. The continuous method renews the chip by injecting the sample and the PBS buffer solution alternatively and injecting SDS-HCl after detection. The method can increase the detection times and extend the life cycle of the biological chip, which is suitable for detecting anti-Clenbuterol, screening anti-Clenbuterol, studying immunoreaction kinetics and establishing standard curves. In addition, the rapid method can adjust the scan angle dynamically in the process of biological chip preparation and immune response detection, which can effectively improve the sensitivity of the detection, remove the redundant data simultaneously, and achieve the detection limit as high as 2 mg/L. Clenbuterol sample (2.75 μg/L) extracted from pork was detected. The proposed methods showed high sensitivity, user-friendly control, rapid detection, label-free analysis, low cost, simple construction, and environmentally friendly equipment. Both methods could achieve the real-time detection of substantial samples.
    35  Comparative Analysis of Volatile Components in Stevia Dry Leaves and its Tea Products
    XIA Yan-bin DENG Zuo
    2013, 29(11):2752-2756.
    [Abstract](1322) [HTML](0) [PDF 539.77 K](682)
    Abstract:
    The composition of aroma compounds in dried Stevia leaf and tea products were analyzed by headspace solid-phase microextraction and gas chromatography-mass spectrometry (SPME-GC-MS) combining with computer search. The results showed that 76 and 73 volatile compounds existed in stevia leaves and tea products respectively. β-Elemene,α-bergapten, (E)-β-farnesene,α-Humulene and Trans nerolidol were identified in both two kinds of dry leaves, but their relative contents were considerable different in the two kinds of samples. In addition, caryophyllene and elixene were the characteristic compounds in stevia dry leaves. Cedrenol, dihydroactinidiolide and (-)-isocaryophyllene were found in stevia tea products. Compared with volatile components of dry leaves in stevia, the types and contents of olefins compounds decreased, while aldehyde compounds, lipids, alcohol compounds increased in its tea product, indicating that rapid heating could enhance the aroma component contents.
    36  Determination of Nine Fluorescent Whitening Agents in Food Contact Polystyrene Materials by High Performance Liquid Chromatography
    WU Zhong-ling CHEN Shu-dong LIN Xiao-jia MU Tong-na WU Zhi-jiang GUO Xin-dong GUO Mao-zhang XIAN Yan-ping LUO Dong-hui
    2013, 29(11):2757-2761.
    [Abstract](1363) [HTML](0) [PDF 511.04 K](653)
    Abstract:
    A new analytical method was developed for the simultaneous determination of nine fluorescent whitening agents (FWA52, FWA135, FWA184, FWA185, FWA199, FWA367, FWA368, FWA378 and FWA393) in food contact polystyrene materials by high performance liquid chromatography-fluorescence detector (HPLC-FLD). The sample was extracted with trichloromethane, and then cleaned up by precipitating the polymer with methanol and analyzed by HPLC using a Phenomenex Gemini C18 column with methanol - water as the mobile phase for gradient elution. The result indicated that the limits of quantification (LOQs) of nine FAWs were ranged from 1.0~8.0 μg/kg and the linear correlation coefficients (r>0.9992) were obtained within the irrespective linear ranges (0.32~200 μg/L). The recovery rates from spiked samples at the spiking levels of 5~500 μg/kg were ranged from 88.0% to 98.9% with RSD of 2.11~4.34% (n=6). The method was simple, precise and had high recovery rate for determination of FAWs in food contact polystyrene materials.
    37  Dielectric Deection of Salted Cucumber treated with Vacuum Acetic Impregnation
    JIN Ya-mei YANG Na ZHANG Jin-fang CHEN Hai-ying JIN Zheng-yu XU xue-ming
    2013, 29(11):2762-2766.
    [Abstract](1034) [HTML](0) [PDF 464.67 K](589)
    Abstract:
    The traditional indicators of pickled cucumber for vacuum acetic impregnation include acidity, texture profile, salinity and nitrite content. The dielectric properties of vacuum impregnated pickled cucumber were measured at 100 kHz~12 MHz frequency range from different time using a fast nondestructive method with parallel-plate capacitor. The results showed that both dielectric constant and loss factor of vacuum-impregnated pickled cucumber decreased monotonically as frequency increased. Negative correlations between dielectric parameters and vacuum impregnated time were observed. Dielectric parameters presented positive correlations with salt and nitrite contents, but no obvious relationship were remarked with acidity and texture profile. At 2.26 MHz and 12 MHz, R2 of the linear regression equations for dielectric constant and salt content were 0.9813 and 0.9858, respectively. Dielectric measurement provided new information concerning pickled vegetables for vacuum impregnation.
    38  Determination of the Residues of Fenamidone and MPID in Animal Source Foods by HPLC-MS
    HUANG Xiu-li NING Huan-yan HUANG Fei CHEN Jia-cong LIANG Shao-cheng HU Kun FENG Xia-ping
    2013, 29(11):2767-2771.
    [Abstract](1142) [HTML](0) [PDF 639.70 K](681)
    Abstract:
    The samples were extracted with acetonitrile, purified by activated PS-DVB and carbon column, and analyzed on C18 column by using a gradient elution using acetonitrile (A)- 5 mmol/L ammonium acetate methanol aqueous solution (B) (V:V=40:60) as mobile phase. The calibration curve presented a good linear relationship between the peak areas and concentrations in the range of 0.05~0.8 μg/mL fenamidone and 0.5~8.0 μg/mL MPID, with correlation coefficients more than 0.999. The average recoveries of standard addition at this concentration level ranged from 82.09% to 109.16%, and the relative standard deviation (RSD) was in the range of 2.46% to 7.89%. The limits of quantification of fenamidone and MPID in animal source foods were 2.0 μg/kg and 20.0 μg/kg. It was suitable for determination of fenamidone and MPID residues in animal source foods with high sensitivity and selectivity.
    39  Determination of Prodigiosin in Fermentation Broth by HPLC
    XIA Yong-jun AI Lian-zhong
    2013, 29(11):2772-2776.
    [Abstract](1425) [HTML](0) [PDF 499.88 K](804)
    Abstract:
    Prodigiosin is a multifaceted secondary metabolite with tripyrrole structure, which attracts great interest for its anticancer activities. At present, the main detection method is spectrophotometry. In order to detect prodigiosinin fermentation broth accurately and rapidly, a high performance liquid chromatography(HPLC)method with gradient elution was developed. The best conditions were established as follows: the separation performed on sepax Bio-C18 column (150 mm×4.6 mm, 3 μm) at 35 ℃ with flow rate of 0.6 mL/min. Under the chosen conditions, prodigiosin could be separated well and showed a symmetrical peak shape with acetonitrile-water gradient elution system. The liner range of prodiogisin was 6~150 μg/mL, and the limit of detection was 0.0058 μg/mL. The recovery of standard addition was 95.74~98.41% with precision of 0.92% relative standard deviation (RSD). The proposed HPLC method could be used for the determination of prodigiosinin fermentation broth with high sensitivity, good repeatability and accuracy.
    40  Water-holding Capacity and Water Content of Myofibrillar Protein Gel by Low-field Nuclear Magnetic Resonance
    LI Yin LI Xia ZHANG Chun-hui SUN Hong-mei DONG Xian-bing XIE Xiao-lei WANG Chun-qing
    2013, 29(11):2777-2781.
    [Abstract](1415) [HTML](0) [PDF 494.30 K](1118)
    Abstract:
    Low-field nuclear magnetic resonance (NMR) and traditional drying method were analyzed to determine water holding capacity (WHC) and water content of myofibrillar protein (MP) gel. The results showed that determination of the spin-spin relaxation time (T2) of MP gel could reflect the compositions and distributions of the three states of water (bound water, immobile water and free water) in MP gel. The higher the free water percentage, the lower the WHC and the higher the water content. The proton density images of MP gels were obtained by magnetic resonance imaging (MRI), which could reflect the content and spatial distribution of water in MP gels. The higher the grey value of proton density image, the higher the water content of MP gel. Pearson’s correlation analysis showed that the grey value, the contents of immobile and free water that measured by low-field NMR were significantly correlated with the results that measured by the traditional drying methods (p<0.05), and the correlation coefficient were 0.97, 0.96 and 0.96, respectively. In conclusion, the low-field NMR technique can be used to determine WHC and water content of MP Gel.
    41  Application of Resonance Light Scattering Spectrometry in Assessment of the Potential Toxicity of Bergapten
    WANG Qian-qian LI Jun-sheng HUANG Guo-xia YAN Liu-juan
    2013, 29(11):2782-2787.
    [Abstract](973) [HTML](0) [PDF 563.07 K](759)
    Abstract:
    DNA intercalation theory was applied in study of the interaction between bergapten which was one kind of flavourings and DNA both in E. coli cells and in vitro by resonance light scattering spectroscopy. The DNA saturation binding value with bergapten was calculated and compared with the saturation values of ethidium bromide, adriamycin, mitoxantrone, and isopsoralen. The results showed that bergapten could partly enter E. coli cells and its abilities to both enter the cells and combine with DNA were lower than those of ethidium bromide. In a neutral environment, the DNA-binding saturation value of bergapten was 0.18, while the saturation values with ethidium bromide, adriamycin, mitoxantrone and isopsoralen were 14.70, 10.58, 3.31, and 0.34, respectively. As a consequence, it appeared that the potential toxicity of bergapten was much lower than those of ethidium bromide, adriamycin, mitoxantrone and a little lower than that of isopsoralen. This research provided technical assistance and reference for quickly testing the potential toxicity of bergapten and flavourings with the similar structure of bergapten.
    42  Distinguishment of Goat Meat Geographical Origin Based on Amino Acids Composition
    LIU Xing-yong LIN Tao LIU Hong-cheng SHAO Jin-liang HE Li-zhong LI Qi-wan CHEN Xing-lian WANG Lu-xiang
    2013, 29(11):2788-2792.
    [Abstract](1085) [HTML](0) [PDF 509.21 K](613)
    Abstract:
    The feasibility of amino acids content analysis was studied for determination of the geographical origin of goat meat. The contents of 17 amino acids were detected in 50 hydrolyzed goat meat samples from five regions of Yunnan Province. By using analysis of variance (ANOVA), principal component analysis (PCA), cluster analysis (CA), K Nearest Neighbor (KNN) analysis and Artificial Neural Networks (ANN). The results showed that, except Cys, the amino acids contents were all different in the samples from different places. Especifically, all the amino acids contents besides Ala and Pro were the highest in the samples from Luoping area, while Ala and Met were the highest amino acid in those from Shilin area. And the content of Pro was the highest in those from Lijiang area and Kunming area. The Kunming and Longling samples had lower amino acids contents than the other samples , and these samples from the two sources showed significant differences (P<0.05) in Glu, Phe, Lys, Arg and Pro contents.. PCA and CA classified the samples into different categories, which was almost consistent to the geographical origin. The KNN accuracy with cross-validation test reached 78.58% in Kunming samples, 100% in Lijiang samples 100% in Shilin samples as well as 93.48% in all samples. ANN provided the best results in classifying geographical origin, showing 100% accuracy in samples from Kunming, Lijiang and Shilin. Therefore, it is feasible to determine the geographical origin of goat meat by the amino acids content analysis.
    43  Application of Proteomics in Safety Assessment and Monitoring of Food Microorganisms
    WU Hui FENG Guang-li LI Xiao-feng LIU Hong-wei
    2013, 29(11):2793-2799.
    [Abstract](2918) [HTML](0) [PDF 561.44 K](695)
    Abstract:
    Food microbial-safety has attracted much attention in the world. Salmonella Spp, Listeria monocytogenes, E.coli O157:H7 can survive in food processing, causing health hazards to consumers. Nowadays, rapid detection of food pathogens and microbial toxins are considered to be important to ensure food safety in the fast food circulation. As one of the six major techniques in life sciences of the 21st Century, proteomics provide new route for safety assessment and monitoring food microbes, which might complement the shortages of the rapid detection methods based on immunology or genomics. This paper mainly discussesed the application of proteomics on inspections of food microbial-safety, including identification of potential pathogenic microorganisms in foods by peptide fingerprints, analysis of the adaption of microorganisms to external environments, etc. Advanced studies on biofilm with proteomics method were also covered in this paper.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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