Extraction of Polysaccharide from Tuber panzhihuanense and its Protective Effect on Oxidative Damage of Liver in Exercise Fatigue Mice
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Abstract:
The extraction process of polysaccharides was optimized by single factor and response surface methodology, and relevant biochemical indicators were measured to explore the protective effect of TPP on oxidative damage of the liver in exercise fatigue mice. The results showed that the optimal extraction process for TPP was ultrasound power 195 W, ultrasound time 30 min, extraction temperature 65 ℃, and liquid-solid ratio 4:1 (mL/g). Under these conditions, the polysaccharide yield was (5.89±0.14)%; In addition, the results of exercise fatigue test in mice showed that compared with the overtraining model group, different doses of TPP significantly decreased the liver index of mice (P<0.05), improved the cellular structure of liver tissue, and significantly reduced the levels of ALT and AST in serum (P<0.05), At the same time, it significantly decreased the content of ROS and lipid peroxide MDA in liver tissue (P<0.05), and significantly increased the activity of SOD, CAT, GSH in liver tissue (P<0.05), and the higher the dose of TPP, The more significant the effect was. The results showed that TPP could enhance the activity of antioxidant enzymes in mouse liver tissue, and its protective effect on liver injury in fatigue mice may be related to reducing the level of oxidative stress in cells.
