Comparison of Four Methods for the Determination of Polysaccharides in Vine Tea (Ampelopsis grossedentata)
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Abstract:
The accuracy of the phenol-sulfuric acid method, the anthrone-sulfuric method, the sulfuric acid-UV method, and the dinitrosalicylicacid colorimetric method for the determination of the polysaccharides in vine tea (Ampelopsis grossedentata) was investigated. The key detection conditions of the four methods were optimized by the single factor test. The accuracy, repeatability, stability, recovery rates of the four methods were compared with the optimal experimental conditions for the determination of polysaccharides in vine tea samples. The results showed that the phenol-sulfuric acid method was the most effective for the determination of polysaccharides in vine tea. The optimal UV detection wavelength was 485.5 nm, and the absorbance at 0.1~0.6 mg/mL with glucose as the standard had a good linear relationship. Under the optimal conditions of 500 μL concentrated sulfuric acid, 600 μL 5% phenol solution, 60 ℃ water bath temperature, and 20 min water bath time in 0.5 mL crude polysaccharide solution (concentration 0.5 mg/mL) of vine tea, the phenol-sulfuric acid method exhibited the highest precision (RSD 0.18%) and the best stability (RSD 0.30%). The results showed that the polysaccharide content of young and old stems and leaves was 9.87~10.47 and 29.77~30.47 mg/g, respectively.
