Metabolites and Pathway Analysis of Barley Seedling Powder under Lead Stress Based on LC-MS Metabolomics
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Abstract:
By simulating barley cultivation in Pb-contaminated area, this study used liquid chromatography-mass spectrometry (LC-MS) metabolomics technology, combined with multivariate statistical analyses such as principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and hierarchical clustering analysis to explore the metabolic pathway of Pb in barley and its influence on the dynamic changes of barley metabolites. Two groups of samples of barley at seedling stage (CMQ) and Pb-stressed CMQ (CMQPb) were selected in the experiment. A total of 84 metabolites were identified, and 60 differential metabolites were obtained by screening the significant metabolites (p<0.05). It was found that Pb stress had a great influence on the contents of organic acids and their derivatives, polyphenols, polyamines, lipids and lipid-like molecules in barley. These results indicate that Pb stress had a certain impact on the nutritional components of barley seedlings. Through the analysis of KEGG metabolic pathway, it was found that Pb stress had the greatest impact on β-alanine metabolism. The four differential metabolites of β-alanine, dihydrouracil, pantothenic acid and uracil are of great significance for the differential metabolic analysis of barley under Pb stress.
