Antibacterial activity of chitosan composite preservative against Pseudomonas fluorescens
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Abstract:
In this experiment, 1.5% chitosan, 0.14% ε-polylysine and 0.15% Sodium D-isoascorbate were used to form a composite preservative, and Pseudomonas fluorescens was used as the experimental strain to investigate the inhibition performance and mechanism of chitosan composite preservative on the dominant bacteria in aquatic products. Methods:the inhibition activity of the preservatives against Pseudomonas fluorescens was investigated by measuring the minimum inhibitory concentration (MIC), inhibition circle and bacterial growth curve,and the changes of OD260 value, ATP and AKP activities, cellular ultrastructure and SDS gel electrophoresis were used to study the antibacterial mechanism of antistaling agent. The results showed that the minimum inhibitory concentration (MIC) of chitosan composite preservative on Pseudomonas fluorescens was 2.24 mg/ml. Chitosan compound antistaling agent had significant antibacterial activity, chitosan compound antistaling agent increased the permeability of cell wall membrane, destroyed the integrity of cell wall membrane, and inhibited the activities of ATP and AKP in bacteria, which were significantly lower than those in the control group(p<0.05). SDS gel electrophoresis showed that the chitosan composite preservative lightened the color of bacterial protein bands and caused the disappearance of some protein bands;Bacterial ultrastructure (SEM) showed that the chitosan composite preservative deformed and ruptured the bacterial body, and a large number of contents flowed out,resulting in the death of the bacteria. This study investigated the mechanism of chitosan composite preservative and proved the inhibition ability, which provides theoretical support for the edible coating preservation of aquatic products.
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Project Supported:
Liaoning Provincial Department of Education 2021 Basic Scientific Research Project (Key Project) (LJKZ0802); Horizontal Project of Jinzhou Medical University (2021-031)
