Establishment of A Bio-barcode Detection Technology for Danofloxacin Residue
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Abstract:
The danofloxacin polyclonal antibody and barcoded DNA strand were simultaneously modified on the surface of gold nanoparticles to prepare gold nanocomposite probe (NP). The magnetic probes (MMPs) were prepared through combining the monoclonal antibody and magnetic particles. After the above two probes are successfully prepared, the hybridization reaction was carried out with the standard, danofloxacin. At the same time, the magnetic field was used to immobilize MMP and remove the unbound NP probe. Finally, the DNA strands labeled on the gold nanocomposite probe were released, and the collected and released DNA strands were detected by microplate silver-stainedbio-barcode technology to obtain indirectly the residual amount of danofloxacin. The detection method does not require expensive equipment, and ordinary laboratories can complete all the experimental operations by using the microplate reader. The operation is simple, the detection is fast, and the detection efficiency is higher than that of traditional ELISA. The results showed that the limit of detection IC15 was 3.62 ng/mL for danofloxacin in the range of 0.05~50 ng/mL, with the detection sensitivity being 100 times that of conventional immunoassay. This method realizes ultra-highly sensitive detection of danofloxacin residue, and can be applied to the rapid detection of other antibiotics. Therefore, the established method is reasonably practicable.
