Comparative Analysis of the Performances of Commercial Composite and Self-made LG Media on Guaranteeing the Sterility of Production Lines
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Abstract:
The sensory and biological characteristics and pH properties of three batches of commercial composite LG media (referred to as the composite LG) and LG media prepared by three beverage companies using raw materials purchased by themselves (referred to as the self-made LG) were compared to evaluate the stability of the composite and self-made LG media and their performances in ensuring the sterility of cold aseptic filling production lines. Results reveal that the three batches of composite LG have similar sensory characteristics, whereas the three self-made LG have markedly different sensory characteristics. As precipitation or impurities are noted, the three media do not meet the appearance standards. Meanwhile, the pH values of the three batches of composite LG are all within the standard range (6.5±0.2), indicating that these media can be used directly without modifying their pH before use; in this regard, they outmatch the self-made LG media. Moreover, the composite LG media give similar culture rates, higher than those of the self-made LG. More specifically, the maximum growth of the eight examined bacteria was noted on the 7th day of culture for the three batches of composite LG, while Aspergillus Niger and Pseudomonas onioniae did not grow in two of the self-made LG media, namely media G and H. Therefore, the following conclusions are drawn: The overall performances of the composite LG are satisfactory, and adequate stability is noted for different batches. Hence, they are suitable for use in cold aseptic filling production lines because they can ensure the sterility of the latter. In contrast, the self-made LG media of different branches show poor stability. Moreover, as low detection sensitivity is observed for all self-made media, the risk of low counts of microorganisms remaining undetected exists when these media are used.
