Protective Effects of an Aqueous Extract of Moringa oleifera Leaves against H2O2-Induced Oxidative Damage in HepG2 Cells
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Abstract:
The antioxidant activity of anaqueous extract of Moringa oleifera leaves was investigated at the cellular level. Taking human hepatoma cells (HepG2 cells) injured by hydrogen peroxide (H2O2)-induced oxidative stress as the model, and the administration with different concentrations of the aqueous extract of Moringa oleifera leaves was for protective intervention. MTT method was used to measure the cell survival rate, and the antioxidant activity indexes such as levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) were determined, to evaluate the protective effect of the aqueous extract of Moringa oleifera leaves against H2O2-induced oxidative damage in cells. The results showed that the survival rate of the HepG2 cells injured by H2O2 increased significantly (p<0.05) by the aqueous extract of Moringa oleifera leaves, with the cell survival rate recovered almost 100% athigh concentrations of the aqueous Moringa oleifera leaf extract (500 and 600 μg/mL). In addition, compared with the model group, the administration of the aqueous extract of Moringa oleifera leaves increased significantly (p<0.05) the activities of GSH-Px and SOD and decreased significantly (p<0.05) the MDA contents in a dose-dependent manner: increase by 20.77%~151.74% for GSH-Px, increase by 22.24%~139.07% for SOD, and decrease by 12.51%~34.04% for MDA. In summary, the aqueous extract of Moringa oleifera leaves has certain antioxidant activity at the cellular level, which can provide a reference for the development of antioxidant products of Moringa oleifera leaves.
