Acer truncatum Bunge Seed Oil Attenuated the Lipopolysaccharide-induced Intestinal Inflammation in Mice
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Abstract:
The effect and mechanism of Acer truncatum Bunge seed oil on lipopolysaccharide (LPS, 3.5 mg/kg) intraperitoneal injection induced intestinal inflammation in male ICR mice were investigated. After 7 days, Acer truncatum Bunge seed oil was administrated by gavage, and corn oil was used as the control. The body weight and colon tissue of mice were observed. The levels of cytokines, including IL-1β, IL-6, IL-18 and TNF-α in serum and colon were measured by ELISA assay, respectively. The mRNA levels of Nlrp3, Asc, Caspase1 and Il1β in colon were detected by qRT-PCR assay. Acer truncatum Bunge seed oil can significantly inhibit the weight loss and reduce the ratio of colon weight to length in LPS-treated mice (p<0.05). At the same time, the levels of IL-1β (155.66 ng/L and 188.85 ng/L), IL-6 (75.42 ng/L and 57.88 ng/L), IL-18 (90.31 ng/L and 52.29 ng/L) and TNF-α (47.97 ng/L and 85.69 ng/L) in serum and colon of the model mice were significantly reduced by the intervention of Acer truncatum Bunge seed oil. The levels of MPO and MDA in colon of model mice were significantly reduced to 81.53 ng/L (vs injury group: 113.59 ng/L, p<0.05) and to 1.04 µmol/g protein (vs injury group: 1.60 µmol/g protein, p<0.05). Compared with the injured group, Acer truncatum Bunge seed oil inhibited the mRNA expression of Nlrp3 (41.80%), Asc (49.85%), Caspase1 (31.28%) and Il1β (39.83%). These results suggest that Acer truncatum Bunge seed oil improve the intestinal inflammation in LPS-treated mice. The mechanism may be associated with the reducing the activation of NLRP3 by Acer truncatum Bunge seed oil in LPS-treated mice.
