Immunoregulatory Effects and Mechanisms of Wheat Bran Arabinoxylan
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
This study aims to investigate the immunomodulatory effects of arabinoxylan (AX) from wheat bran on mouse peritoneal macrophages and the relevant mechanisms. The effects of AX at different concentrations on the release of nitric oxide (NO), as well as cell viability of mouse peritoneal macrophages, were studied. Mouse peritoneal macrophages were treated with AX for 0, 1, 3, and 6 h to observe the effects of AX on the expressions of immune-associated genes and proteins related to MAPK and Akt signaling pathways. The results show that AX at concentrations of 6.25 and 12.5 μg/mL have no significant toxicity to mouse peritoneal macrophages, and the cell survival rates are 90.42% and 89.99%, respectively (p>0.05). However, when the concentration exceeds 12.5 μg/mL, the cell survival rate is significantly reduced (p<0.05). Comparisons between the control and treatment group demonstrate that AX at 6.25~200 μg/mL can significantly increase NO release (p<0.05). Moreover, the expressions of immune-associated gene mRNAs (1L-1β, IL-6, TNF-α, iNOS, COX-2, and Nfkbia) are enhanced. The phosphorylation levels of MAPK and Akt pathway proteins (ERK1/2, JNK, p38 MAPK, and Akt) are also increased by AX. These results suggest that AX can regulate immunity by activating mouse peritoneal macrophages, increasing the production of NO, enhancing the mRNA expressions of immune-associated genes, and activating MAPK and Akt signaling pathways.
