The Active Components of Testosterone Secreted by Antler Testis Stromal Cells Rapidly Screened by Electrospray Tandem Mass Spectrometry and Chemometrics
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Abstract:
Electrospray tandem mass spectrometry (ESI-MS/MS) and chemometrics were used to screen the active components of testosterone secreted by testis stromal cells of Antler antler. Using electrospray tandem mass spectrometry, the chemical composition of 6 batch of pilose antler and 6 batch of velvet antler prepared by traditional process was detected. The testosterone secretion of rat leydig cells was also determinated. The principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the mass spectrometry data. Rapid differentiation of fresh velvet antler and traditional processed products was achieved. Partial least square method (PLS) and gray correlation degree analysis (GRA) were used to select the active components with potential testosterone secretion by testicular stromal cells. The structure and composition of the possible active components were determined by secondary mass spectrometry data analysis. The results showed that the components of fresh antler and traditional processed products were significantly different, and both of them had good activity of testosterone secretion by testicular stromal cells. The most active components were Tyr-Ser-Phe (m/z 416), PHE-Phe-Leu/Ile (m/z 426), respectively. This method could be used to analyze the active components of testicular stromal cells in antler and provide reference for the development of antler as health food.
