Antifungal Activities of Extracts and Chemical Constituents Analysis of N-butanol Extract from Phellodendron chinense
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Abstract:
The antifungal activity and chemical components of the extracts of Phellodendron chinense with the strongest antifungal effect were investigated to provide the foundation for the development of new botanical fungicides. The extract of Phellodendron chinense was prepared by ultrasonic-assisted ethanol extraction, and then extracted with equal volumes of petroleum ether, chloroform, ethyl acetate, and n-butanol, respectively. The antifungal activity of each extract was determined by means of the mycelium growth rate method. The chemical components of the extract with the strongest antifungal effect were analyzed by GC-MS. The results indicated that n-butanol extract had the strongest inhibitory effect, with an antifungal rate of 84.46%, a minimum inhibitory concentration (MIC) of 20 mg/mL, and a half maximal inhibitory concentration (EC50) value of 6.480 mg/mL, respectively. GC-MS analysis results showed that 26 compounds were separated and identified from n-butanol extract, mainly alkaloids, organic acids, glycosides and amides. The component with the highest relative content was 5-(2-Thienyl)-4-pyrimidinamine, accounting for 52.85%. The antifungal active ingredients of extracts of Phellodendron chinense branches and barks were mainly present in the n-butanol part, and were dominated by the alkaloids compound. It was supposed that the alkaloids compound was the main antifungal active ingredient of Phellodendron chinense branches and barks in inhibition of fungal diseases of plants, providing the foundation and reference basis for development and application of botanical fungicides.
