Inhibitory Effect of Selenium-rich Protein in Green Tea on HepG2 Cells of Liver Cancer
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Abstract:
The inhibitory effect of the selenium-rich proteins in green tea on HepG2 cells of live cancer was analyzed in this study. HepG2 cells in the logarithmic growth stage were incubated in the DEEM medium and used as a control group. The selenium-rich proteins of green tea at concentrations of 0.11 g/L, 0.18 g/L, 0.25 g/L and 0.5 g/L, respectively, were used as the experimental groups. The DMEM medium supplemented with dimethyl sulfoxide (DMSO) was used as the reference group. The inhibition rate was calculated by the optical density value, the apoptotic rate was detected by flow cytometry, and apoptosis-related factors were detected experimentally. The results showed that the inhibitory rate for the HepG2 cells of liver cancer increased with time and the concentration of selenium-rich proteins in green tea, but the inhibition rate for HepG2 cells for the control group was always zero. The apoptotic rate for the HepG2 cells of the control group was 6.17%, and the maximum apoptotic rate reached the maximum (30.60%) when the concentration of selenium-rich proteins of the green tea was 0.5 g/L. The expression level of apoptosis factors of the experimental groups showed an overall downward trend, the Bcl-2 and Bax ratio was lower than 1, indicating that the higher the concentration of the selenium-rich proteins of green tea, the longer the incubation time, the stronger the ability to inhibit HepG2 tumor cells, selenium-rich proteins. Therefore, green tea’s selenium-rich proteins can significantly decrease the number of S-phase cells, which indicates that the green tea selenium-rich proteins can inhibit HepG2 cells of liver cancer.
