Analysis of the Effects of Angelica sinensis Ultrafine Powder on the Diversity of Intestinal Flora in Mice Based on 16S rRNA Technology
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The effects of different doses of ultrafine powder on the diversity of intestinal flora and physiological structure of the intestinal tract in mice were studied. C57BL/6 mice were divided into high-dose ultrafine powder group (2.0 g/kg), medium-dose group (1.0 g/kg), low-dose group (0.5 g/kg) and traditional decoction piece group (2.0 g/kg), the traditional powder group (2.0 g/kg) and the blank group, and subject to intragastric administration for 15 days. The body weights were recorded. On the 15th day of gavage, the colon contents were taken out aseptically, the V3+V4 region of the 16s rRNA gene of the intestinal flora was amplified, and the beta diversity and alpha diversity were analyzed by high-throughput sequencing. The compositions of the species at the phylum level and genus level were analyzed, and the pathological changes of the gastrointestinal tract was examined by HE staining. The results showed that the body weight of C57BL/6 mice grew normally. The high-dose of Angelica sinensis ultrafine powder decoction pieces significantly increased the content of Verrucomicrobia (p<0.05) and decreased the content of Actinobacteria (p<0.01). In addition, in the medium-dose group of Angelica sinensis ultrafine powder decoction pieces, the content of Bacteroidetes decreased the most with the most significant increase of Firmicutes. At the genus level, the medium-dose increased the most significantly the content of Faecalibaculum (p<0.05). Therefore, the medium dose of ultrafine powder was the most suitable experimental dose. There was no pathological change in the gastrointestinal tract of mice in each treatment group. This experiment can provide a theoretical basis for the scientific dosage and safety of Angelica sinensis ultrafine powder for human health care or treatment, and offer technical support for the effectiveness of the intestinal flora.
