Study on the Effect of Jupi Decoction on the Structure of Intestinal Flora Based on SHIME
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Abstract:
This study was based on the simulator of the human intestinal microbial ecosystem (SHIME), and human intestinal microorganisms were introduced to simulate in vitro the human microbial ecology. The effects of jupi decoction on intestinal flora were examined by 16S rRNA high-throughput sequencing technology, and the effects of jupi decoction on the metabolites of intestinal microflora, short-chain fatty acids (SCFAs), were investigated by gas chromatography. The results showed that after the intervention of jupi decoction, the relative abundance of Veronica decreased by 33.65% during the intervention period and by 92.78% during the maintenance period. The relative abundance of Klebsiella decreased by 63.60% and 67.82%, respectively, during the intervention and maintenance periods, while that of Macromonas increased by 27.5% and 62.61% during the intervention and maintenance periods, respectively. The content of acetic acid among the short chain fatty acids decreased first and then increased (by 3.21% in general), while the content of propanoic acid decreased by 45.43% (p<0.01). The contents of isobutyric acid, butyric acid, isovaleric acid and valeric acid increased by 52.94%, 40.86%, 48.94% and 80.00%, respectively. The intervention with jupi decoction can change the diversity of intestinal microflora, regulate the level of microbial abundance, exhibit inhibitory effects on the growth of harmful intestinal microorganisms such as Veronica and Klebsiella, and promote the production of intestinal microbial metabolites, short chain fatty acids, which improves effectively the structure of human intestinal flora.
