Determination of Organotin Pesticide Residues in Fruits and Vegetables by Gas Chromatography-tandem Mass Spectrometry with Isotope-labeled Internal Standards
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Abstract:
A gas chromatography-tandem mass spectrometric (GC-MS/MS) method with isotope-labeled internal standards was developed for the determination of organotin pesticide residues such as cyhexatin, azocylotin, fentin-acetate, triphenyltin hydroxide and fenbutatin oxide in vegetables and fruits. Samples were extracted with 1% glacial aceticacid-acetonitrile, derivatized with sodium tetraethyl borate, purified with a PC/NH2-SPE column, eluted with n-hexane-acetone (99:1, V/V), and determined using an EI source. The results showed that good linearity was found in the concentration range of 0.05~1.00 mg/L for the determination of the three organotin pesticides, with all the correlation coefficients higher than 0.998. The recovery rates of organotin ranges in the negative samples spiked at the three levels (0.04, 0.10 and 0.20 mg/kg) ranged from 81.64% to 106.23%, with the relative standard derivations (RSDs) all lower than 6.33% (n=6). The limits of quantification (LOQ) of the proposed method for cyhexatin, fentin-acetate, and fenbutatin oxide were 0.01, 0.009 and 0.039 mg/kg, respectively. This method is stable, reliable and highly sensitive, thereby suitable for the determination of organotin pesticide residues in agricultural products such as fruits and vegetables. This method also provides a reference for the determination of organotin pesticide residues in other foods.
